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Product Info Summary
| SKU: | M02099 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Securin Monoclonal Antibody
SKU/Catalog Number
M02099
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Securin Monoclonal Antibody catalog # M02099. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Securin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02099)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADFH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02099 is reactive to PTTG1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
58 kDa
Calculated molecular weight
16320 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02099 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Securin expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For PTTG1 (Source: Uniprot.org, NCBI)
Gene Name
PTTG1
Full Name
Securin
Weight
16320 MW
Superfamily
securin family
Alternative Names
Actin-related protein 2/3 complex subunit 5;Arp2/3 complex 16 kDa subunit;p16-ARC;ARPC5;ARC16; PTTG1 EAP1, HPTTG, PTTG, TUTR1 PTTG1 regulator of sister chromatid separation, securin securin|ESP1-associated protein 1|pituitary tumor-transforming 1|pituitary tumor-transforming gene 1 protein|tumor-transforming protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTTG1, check out the PTTG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTTG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Securin Monoclonal Antibody (M02099)
Hello CJ!
M02099 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu XM,Chen F,Wang L,Zhang F,Huo LJ.Checkpoint kinases are required for oocyte meiotic progression by the maintenance of normal spindle structure and chromosome condensation.Exp Cell Res.2021 May 31:112657.doi:10.1016/j.yexcr.2021.112657.Epub ahead of print. PMID:34081985.
Species: Mouse
M02099 usage in article: APP:IF, SAMPLE:OOCYTES, DILUTION:1:50
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Anti-Securin Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02099?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Securin expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Can you help my question with product M02099, anti-Securin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02099 anti-Securin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
Is there a BSA free version of anti-Securin Monoclonal antibody M02099 available?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Securin Monoclonal antibody M02099 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-25
Question
See below the WB image, lot number and protocol we used for secondary oocyte using anti-Securin Monoclonal antibody M02099. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-28
Question
Does anti-Securin Monoclonal antibody M02099 work on goat WB with pituitary adenoma?
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
Our lab technicians have not validated anti-Securin Monoclonal antibody M02099 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Securin Monoclonal antibody M02099 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat pituitary adenoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-06
Question
Is this M02099 anti-Securin Monoclonal antibody reactive to the isotypes of PTTG1?
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
The immunogen of M02099 anti-Securin Monoclonal antibody is A synthesized peptide derived from human Securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-22
Question
Our lab want to know about to test anti-Securin Monoclonal antibody M02099 on human secondary oocyte for research purposes, then I may be interested in using anti-Securin Monoclonal antibody M02099 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Johnson
Verified customer
Asked: 2017-04-19
Answer
The products we sell, including anti-Securin Monoclonal antibody M02099, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-19
Question
I see that the anti-Securin Monoclonal antibody M02099 works with IP, what is the protocol used to produce the result images on the product page?
R. Walker
Verified customer
Asked: 2014-09-02
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-09-02
