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Product Info Summary
| SKU: | A09726-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SEC24A Antibody Picoband®
SKU/Catalog Number
A09726-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEC24A Antibody Picoband® catalog # A09726-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SEC24A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09726-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SEC24A recombinant protein (Position: Q43-Q1045).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09726-1 is reactive to SEC24A in Human, Monkey
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
67169 MW
Background of SEC24A
SEC24 family, member A (S. cerevisiae) is a protein that in humans is encoded by the SEC24A gene. The protein encoded by this gene belongs to a family of proteins that are homologous to yeast Sec24. This protein is a component of coat protein II (COPII)-coated vesicles that mediate protein transport from the endoplasmic reticulum. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the golgi complex. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09726-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Monkey
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human 292T whole cell, human HepG2 whole cell, monkey COS-7 whole cell
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SEC24A using anti-SEC24A antibody (A09726-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 292T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC24A antigen affinity purified polyclonal antibody (Catalog # A09726-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC24A at approximately 120 kDa. The expected band size for SEC24A is at 66 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-SEC24A antibody (A09726-1).
Overlay histogram showing HEL cells stained with A09726-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC24A Antibody (A09726-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SEC24A (Source: Uniprot.org, NCBI)
Gene Name
SEC24A
Full Name
Protein transport protein Sec24A
Weight
67169 MW
Superfamily
SEC23/SEC24 family
Alternative Names
Cationic amino acid transporter 3;CAT-3;CAT3;Cationic amino acid transporter y+;Solute carrier family 7 member 3;SLC7A3;ATRC3, CAT3; SEC24A SEC24 homolog A, COPII coat complex component protein transport protein Sec24A|SEC24 family, member A|SEC24 related gene family, member A|SEC24-related protein A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEC24A, check out the SEC24A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEC24A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEC24A Antibody Picoband® (A09726-1)
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