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Product Info Summary
| SKU: | PA1718 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SDHB Antibody Picoband®
SKU/Catalog Number
PA1718
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SDHB Antibody catalog # PA1718. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDHB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1718)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SDHB, different from the related rat sequence by one amino acid and mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1718 is reactive to SDHB in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
31630 MW
Background of SDHB
SDHB (Succinate Dehydrogenase Complex, Subunit B, iron sulfur protein), also known as iron-sulfur subunit of complex II (Ip) or SDH2, HOMOLOG OF, is a protein that in humans is encoded by the SDHB gene. SDHB is one of four protein subunits forming succinate dehydrogenase, the other three being SDHA, SDHC and SDHD. The SDHB subunit is connected to the SDHA subunit on the hydrophilic, catalytic end of the SDH complex. The SDHB gene is mapped on 1p36.13. The entire SDHB transcript is encoded by 8 exons within approximately 40 kb by Au et al (1995). Pollard et al. (2005) stated that the nuclear-encoded Krebs cycle enzymes fumarate hydratase and succinate dehydrogenases like SDHB act as tumor suppressors, and germline mutations in these genes predispose individuals to leiomyomas and renal cancer and to paragangliomas, respectively. In affected members of families with paragangliomas-4, mutations were identified in the SDHB gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1718 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue
IHC: human liver cancer tissue, human lung adenocarcinoma tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SDHB using anti-SDHB antibody (PA1718).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHB antigen affinity purified polyclonal antibody (Catalog # PA1718) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo
r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHB at approximately 29 kDa. The expected band size for SDHB is at 29 kDa.
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Figure 2. IHC analysis of SDHB using anti-SDHB antibody (PA1718).
SDHB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (PA1718) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SDHB using anti-SDHB antibody (PA1718).
SDHB was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (PA1718) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SDHB (Source: Uniprot.org, NCBI)
Gene Name
SDHB
Full Name
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Weight
31630 MW
Superfamily
succinate dehydrogenase/fumarate reductase iron-sulfur protein family
Alternative Names
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial;1.3.5.1;Iron-sulfur subunit of complex II;Ip;SDHB;SDH, SDH1; SDHB CWS2, IP, MC2DN4, PGL4, SDH, SDH1, SDH2, SDHIP succinate dehydrogenase complex iron sulfur subunit B succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial|iron-sulfur subunit of complex II|succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SDHB, check out the SDHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDHB Antibody Picoband® (PA1718)
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No publications found for PA1718
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Customer Q&As
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17 Customer Q&As for Anti-SDHB Antibody Picoband®
Question
Is this PA1718 anti-SDHB antibody reactive to the isotypes of SDHB?
D. Singh
Verified customer
Asked: 2020-03-26
Answer
The immunogen of PA1718 anti-SDHB antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SDHB(42-59 FAIYRWDPDKAGDKPHMQ), different from the related rat sequence by one amino acid and mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-26
Question
I am interested in to test anti-SDHB antibody PA1718 on rat liver for research purposes, then I may be interested in using anti-SDHB antibody PA1718 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The products we sell, including anti-SDHB antibody PA1718, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-17
Question
Does anti-SDHB antibody PA1718 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
According to the expression profile of liver, SDHB is highly expressed in liver. So, it is likely that anti-SDHB antibody PA1718 will work for IHC with liver.
Boster Scientific Support
Answered: 2020-03-02
Question
Our lab were satisfied with the WB result of your anti-SDHB antibody. However we have observed positive staining in uterus mitochondrion inner membrane using this antibody. Is that expected? Could you tell me where is SDHB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Based on literature, uterus does express SDHB. Generally SDHB expresses in mitochondrion inner membrane. Regarding which tissues have SDHB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2494655
Liver, Pubmed ID: 2302193, 7622059, 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-27
Question
Please see the WB image, lot number and protocol we used for liver using anti-SDHB antibody PA1718. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
I see that the anti-SDHB antibody PA1718 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-06
Question
Would PA1718 anti-SDHB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
As indicated on the product datasheet, PA1718 anti-SDHB antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-15
Question
My question regarding product PA1718, anti-SDHB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1718 anti-SDHB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-04
Question
We are currently using anti-SDHB antibody PA1718 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-SDHB antibody (PA1718) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
Question
Will anti-SDHB antibody PA1718 work on dog WB with brain?
Verified Customer
Verified customer
Asked: 2019-05-01
Answer
Our lab technicians have not validated anti-SDHB antibody PA1718 on dog. You can run a BLAST between dog and the immunogen sequence of anti-SDHB antibody PA1718 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-01
Question
We ordered your anti-SDHB antibody for WB on uterus a few years ago. I am using human, and We want to use the antibody for IHC next. I would like examining uterus as well as muscle of leg in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
I took a look at the website and datasheets of our anti-SDHB antibody and it appears that PA1718 has been tested on human in both WB and IHC. Thus PA1718 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-01-29
Question
I am interested in using your anti-SDHB antibody for aerobic respiration studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
We appreciate your inquiry. This PA1718 anti-SDHB antibody is validated on rat testis tissue, cardiac muscle tissue, tissue lysate, jurkat whole cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-25
Question
We have seen staining in human uterus. What should we do? Is anti-SDHB antibody supposed to stain uterus positively?
L. Parker
Verified customer
Asked: 2018-10-23
Answer
From literature uterus does express SDHB. From Uniprot.org, SDHB is expressed in muscle of leg, liver, uterus, brain, fibroblast, among other tissues. Regarding which tissues have SDHB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2494655
Liver, Pubmed ID: 2302193, 7622059, 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-10-23
Question
Do you have a BSA free version of anti-SDHB antibody PA1718 available?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SDHB antibody PA1718 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-13
Question
Is a blocking peptide available for product anti-SDHB antibody (PA1718)?
K. Jackson
Verified customer
Asked: 2016-02-03
Answer
We do provide the blocking peptide for product anti-SDHB antibody (PA1718). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-SDHB antibody PA1718. Let me know if you need anything else.
H. Zhang
Verified customer
Asked: 2015-04-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-06
Question
I was wanting to use your anti-SDHB antibody for IHC for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
B. Zhao
Verified customer
Asked: 2014-03-11
Answer
You can see on the product datasheet, PA1718 anti-SDHB antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-11
