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Product Info Summary
| SKU: | A07883-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SCNN1D Antibody
SKU/Catalog Number
A07883-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SCNN1D Antibody (Catalog # A07883-1). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCNN1D Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07883-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SCNN1D.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07883-1 is reactive to SCNN1D in Human
Reconstitution
Calculated molecular weight
70215 MW
Background of SCNN1D
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Waldmann R., J. Biol. Chem. 270:27411-27414(1995).
Ota T., Nat. Genet. 36:40-45(2004).
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07883-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from RAW264.7 cells, using SCNN1D antibody A07883-1.
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Immunofluorescence analysis of A549 cells, using SCNN1D antibody A07883-1.
Protein Target Info & Infographic
Gene/Protein Information For SCNN1D (Source: Uniprot.org, NCBI)
Gene Name
SCNN1D
Full Name
Amiloride-sensitive sodium channel subunit delta
Weight
70215 MW
Superfamily
amiloride-sensitive sodium channel (TC 1.A.6) family
Alternative Names
Amiloride-sensitive sodium channel subunit delta;Delta-NaCH;Epithelial Na (+) channel subunit delta;Delta-ENaC;ENaCD;Nonvoltage-gated sodium channel 1 subunit delta;SCNED;SCNN1D;DNACH; SCNN1D ENaCd, ENaCdelta, SCNED, dNaCh sodium channel epithelial 1 subunit delta amiloride-sensitive sodium channel subunit delta|delta-ENaC|delta-NaCH|epithelial Na(+) channel subunit delta|nonvoltage-gated sodium channel 1 subunit delta|sodium channel epithelial 1 delta subunit|sodium channel, non-voltage-gated 1, delta subunit|sodium channel, nonvoltage-gated 1, delta|sodium channel, voltage-gated, type I, delta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCNN1D, check out the SCNN1D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCNN1D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCNN1D Antibody (A07883-1)
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No publications found for A07883-1
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Customer Q&As
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7 Customer Q&As for Anti-SCNN1D Antibody
Question
I see that the anti-SCNN1D antibody A07883-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-06
Question
Does anti-SCNN1D antibody A07883-1 work for WB with hippocampus testis?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
According to the expression profile of hippocampus testis, SCNN1D is highly expressed in hippocampus testis. So, it is likely that anti-SCNN1D antibody A07883-1 will work for WB with hippocampus testis.
Boster Scientific Support
Answered: 2020-01-20
Question
Will anti-SCNN1D antibody A07883-1 work on pig IF with brain?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Our lab technicians have not tested anti-SCNN1D antibody A07883-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SCNN1D antibody A07883-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-31
Question
Would A07883-1 anti-SCNN1D antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
It shows on the product datasheet, A07883-1 anti-SCNN1D antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-01
Question
Please see the WB image, lot number and protocol we used for hippocampus testis using anti-SCNN1D antibody A07883-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-08
Question
I was wanting to use your anti-SCNN1D antibody for WB for human hippocampus testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus testis identification?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
As indicated on the product datasheet, A07883-1 anti-SCNN1D antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-08
Question
We are currently using anti-SCNN1D antibody A07883-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
K. Anderson
Verified customer
Asked: 2013-12-10
Answer
The anti-SCNN1D antibody (A07883-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-10
