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Product Info Summary
| SKU: | A00592-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-SAMHD1 Antibody
SKU/Catalog Number
A00592-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SAMHD1 Antibody (Catalog # A00592-1). Tested in ELISA, WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SAMHD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SAMHD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00592-1)
Host
Rabbit
Contents
SAMHD1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-SAMHD1 antibody was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human SAMHD1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SAMHD1 antibody is human and mouse reactive.
Reactive Species
A00592-1 is reactive to SAMHD1 in Human, Mouse
Applications
A00592-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
72201 MW
Background of SAMHD1
The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1). SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3). Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot, Immunohistochemistry, Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human Daudi Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: SAMHD1 A00592-1, 1 μg/mL, in (A: the absence and B: the presence of blocking peptide), (1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Overexpression Validation in 293T Transfected Cells
Loading: 15 μg of lysates per lane.Antibodies: SAMHD1 A00592-1 (0.1 μg/mL), (1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.293 cells were transfected with (1) wild-type SAMHD1 or (2) SAMHD1 (mutation T592A).
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Immunohistochemistry Validation of SAMHD1 in Human Brain Tissue
Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using anti-SAMHD1 antibody (A00592-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of SAMHD1 in Human Daudi cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Daudi Cells labeling SAMHD1 with A00592-1 at 20 μg/mL
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KO Validation of SAMHD1 in xenograft mice (Kodigepalli et al., 2018)
THP-1 control and SAMHD1 KO (THP-1/KO) cells were injected into NSG (non-obese diabetic/severe combinedimmune deficient-gamma) mice. Protein expression levels of SAMHD1 were examined by Western blot with anti-SAMHD1 antibodies (A00592-1) and SAMHD1 was not detected in THP-1/KO cells.
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Overexpression Validation of SAMHD1 in CD4+ T-cells from a healthy donor and transformed CD4+ T-cell lines (Kohnken et al.
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Overexpression of SAMHD1 in CD4+ T-cells from Healthy Donors and Sézary Syndrome (SS) patients(Kohnken et al.
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Regulated Expression Validation of SAMHD1 in CD4+ T-cells from a healthy donor (Kohnken et al.
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Regulated Expression Validation of SAMHD1 in MT2 CD4+ T-cells from leukemia patients(Kohnken et al.
Protein Target Info & Infographic
Gene/Protein Information For SAMHD1 (Source: Uniprot.org, NCBI)
Gene Name
SAMHD1
Full Name
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Weight
72201 MW
Superfamily
SAMHD1 family
Alternative Names
AGS5; CHBL2; DCIP; DCIPMg11; Dendritic cell-derived IFNG-induced protein; EC 3.1.4.-; HDDC1; Monocyte protein 5; MOP5; MOP-5; MOP-5AGS5; SAM domain and HD domain 1; SAM domain and HD domain-containing protein 1; SAMHD1; SBBI88 SAMHD1 CHBL2, DCIP, HDDC1, MOP-5, SBBI88, hSAMHD1 SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 deoxynucleoside triphosphate triphosphohydrolase SAMHD1|SAM domain and HD domain 1|SAM domain and HD domain-containing protein 1|dNTPase|dendritic cell-derived IFNG-induced protein|monocyte protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SAMHD1, check out the SAMHD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SAMHD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SAMHD1 Antibody (A00592-1)
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