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Product Info Summary
| SKU: | A01567 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody
View all Ribosomal Protein S6/RPS6 Antibodies
SKU/Catalog Number
A01567
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody (Catalog # A01567). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01567)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01567 is reactive to RPS6 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
28681 MW
Background of Ribosomal Protein S6/RPS6
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
McBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081.
Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097.
Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222.
Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01567 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 32kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using S6 Ribosomal Protein(Ab-235) antibody A01567(left) or the same antibody preincubated with blocking peptide(right).
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Western blot analysis of extracts from HepG2 and 293 cells using S6 Ribosomal Protein(Ab-235) antibody A01567.
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Immunofluorescence staining of methanol-fixed MCF7 cells using S6 Ribosomal Protein(Ab-235) antibody A01567.
Protein Target Info & Infographic
Gene/Protein Information For RPS6 (Source: Uniprot.org, NCBI)
Gene Name
RPS6
Full Name
40S ribosomal protein S6
Weight
28681 MW
Superfamily
eukaryotic ribosomal protein eS6 family
Alternative Names
40S ribosomal protein S6;Phosphoprotein NP33;RPS6;OK/SW-cl.2; RPS6 S6 ribosomal protein S6 40S ribosomal protein S6|phosphoprotein NP33|small ribosomal subunit protein eS6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6, check out the RPS6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody (A01567)
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No publications found for A01567
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody
Question
Is this A01567 anti-S6 Ribosomal Protein(Ab-235) antibody reactive to the isotypes of RPS6?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The immunogen of A01567 anti-S6 Ribosomal Protein(Ab-235) antibody is Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-10
Question
Does anti-S6 Ribosomal Protein(Ab-235) antibody A01567 work for IHC with colon adenocarcinoma?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
According to the expression profile of colon adenocarcinoma, RPS6 is highly expressed in colon adenocarcinoma. So, it is likely that anti-S6 Ribosomal Protein(Ab-235) antibody A01567 will work for IHC with colon adenocarcinoma.
Boster Scientific Support
Answered: 2020-03-27
Question
I have a question about product A01567, anti-S6 Ribosomal Protein(Ab-235) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01567 anti-S6 Ribosomal Protein(Ab-235) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-26
Question
Do you have a BSA free version of anti-S6 Ribosomal Protein(Ab-235) antibody A01567 available?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S6 Ribosomal Protein(Ab-235) antibody A01567 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-11
Question
Is a blocking peptide available for product anti-S6 Ribosomal Protein(Ab-235) antibody (A01567)?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
We do provide the blocking peptide for product anti-S6 Ribosomal Protein(Ab-235) antibody (A01567). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-10
Question
We are interested in to test anti-S6 Ribosomal Protein(Ab-235) antibody A01567 on human colon adenocarcinoma for research purposes, then I may be interested in using anti-S6 Ribosomal Protein(Ab-235) antibody A01567 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The products we sell, including anti-S6 Ribosomal Protein(Ab-235) antibody A01567, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-30
Question
I was wanting to use your anti-S6 Ribosomal Protein(Ab-235) antibody for IHC for human colon adenocarcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon adenocarcinoma identification?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
It shows on the product datasheet, A01567 anti-S6 Ribosomal Protein(Ab-235) antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon adenocarcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon adenocarcinoma using anti-S6 Ribosomal Protein(Ab-235) antibody A01567. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
I was wanting to use using your anti-S6 Ribosomal Protein(Ab-235) antibody for ribosomal scanning and start codon recognition studies. Has this antibody been tested with western blotting on lung carcinoma tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
I appreciate your inquiry. This A01567 anti-S6 Ribosomal Protein(Ab-235) antibody is tested on lung carcinoma tissue, 293 cells, mcf7 cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-12
Question
See attached the WB image, lot number and protocol we used for colon adenocarcinoma using anti-S6 Ribosomal Protein(Ab-235) antibody A01567. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
We have seen staining in mouse connective tissue. What should we do? Is anti-S6 Ribosomal Protein(Ab-235) antibody supposed to stain connective tissue positively?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Based on literature connective tissue does express RPS6. Based on Uniprot.org, RPS6 is expressed in connective tissue, colon adenocarcinoma, colon, muscle, pancreas, skin testis, placenta, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have RPS6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Muscle, Pancreas, Skin, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 8706699
Boster Scientific Support
Answered: 2019-06-25
Question
Would A01567 anti-S6 Ribosomal Protein(Ab-235) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
As indicated on the product datasheet, A01567 anti-S6 Ribosomal Protein(Ab-235) antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-05
Question
I see that the anti-S6 Ribosomal Protein(Ab-235) antibody A01567 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-13
Question
Our lab used your anti-S6 Ribosomal Protein(Ab-235) antibody for IHC on connective tissue a few years ago. I am using mouse, and We want to use the antibody for WB next. you antibody examining connective tissue as well as colon in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
S. Kulkarni
Verified customer
Asked: 2016-05-20
Answer
I have checked the website and datasheets of our anti-S6 Ribosomal Protein(Ab-235) antibody and I see that A01567 has been tested on mouse in both IHC and WB. Thus A01567 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-05-20
Question
We are currently using anti-S6 Ribosomal Protein(Ab-235) antibody A01567 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
O. Evans
Verified customer
Asked: 2013-07-08
Answer
The anti-S6 Ribosomal Protein(Ab-235) antibody (A01567) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-07-08
