Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody

Product Name

Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody

View all Ribosomal Protein S6/RPS6 Antibodies

SKU/Catalog Number

P01567

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody (Catalog # P01567). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01567)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 of mouse rpS6, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Mouse.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01567 is reactive to RPS6 in Human, Mouse

Reconstitution

Observed Molecular Weight

52 kDa

Calculated molecular weight

28681 MW

Background of Ribosomal Protein S6/RPS6

Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P01567 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500

Validation Images & Assay Conditions

Gene/Protein Information For RPS6 (Source: Uniprot.org, NCBI)

Alternative Names

40S ribosomal protein S6 antibody, Air8 antibody, NP33 antibody, Phosphoprotein NP33 antibody, Pp30 antibody, Ribosomal protein S6 antibody, RP S6 antibody, rps6 antibody, RPS6 antibody, RS6 antibody, RS6_HUMAN antibody, S6 antibody, S6 Ribosomal Protein antibody RPS6 S6 ribosomal protein S6 40S ribosomal protein S6|phosphoprotein NP33|small ribosomal subunit protein eS6

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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