Click image to see more details
Product Info Summary
| SKU: | A10314 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Protein S100-A5 S100A5 Antibody
SKU/Catalog Number
A10314
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Protein S100-A5 S100A5 Antibody (Catalog # A10314). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein S100-A5 S100A5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10314)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human S100A5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10314 is reactive to S100A5 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
10744 MW
Background of S100A5
Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.
Engelkamp D., Proc. Natl. Acad. Sci. U.S.A. 90:6547-6551(1993).
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10314 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, using S100A5 antibody A10314.
Protein Target Info & Infographic
Gene/Protein Information For S100A5 (Source: Uniprot.org, NCBI)
Gene Name
S100A5
Full Name
Protein S100-A5
Weight
10744 MW
Superfamily
S-100 family
Alternative Names
Protein S100-A5;Protein S-100D;S100 calcium-binding protein A5;S100A5;S100D; S100A5 S100D S100 calcium binding protein A5 protein S100-A5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100A5, check out the S100A5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100A5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein S100-A5 S100A5 Antibody (A10314)
Hello CJ!
No publications found for A10314
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Protein S100-A5 S100A5 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Protein S100-A5 S100A5 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Protein S100-A5 S100A5 Antibody
Question
I see that the anti-S100A5 antibody A10314 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-13
Question
Does anti-S100A5 antibody A10314 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of kidney, S100A5 is highly expressed in kidney. So, it is likely that anti-S100A5 antibody A10314 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-08-20
Question
Will anti-S100A5 antibody A10314 work on monkey WB with kidney?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Our lab technicians have not tested anti-S100A5 antibody A10314 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-S100A5 antibody A10314 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-14
Question
Does A10314 anti-S100A5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
You can see on the product datasheet, A10314 anti-S100A5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-08
Question
We are currently using anti-S100A5 antibody A10314 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
T. Mangal
Verified customer
Asked: 2018-04-20
Answer
The anti-S100A5 antibody (A10314) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-20
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-S100A5 antibody A10314. Let me know if you need anything else.
T. Lewis
Verified customer
Asked: 2016-06-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-28
Question
My question regarding product A10314, anti-S100A5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Wu
Verified customer
Asked: 2014-08-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10314 anti-S100A5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-08-13
