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Product Info Summary
| SKU: | A02215-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody
SKU/Catalog Number
A02215-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody catalog # A02215-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02215-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Rsk 2/MAPKAP Kinase 1b/RPS6KA3 recombinant protein (Position: D393-K733).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02215-2 is reactive to RPS6KA3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
84 kDa
Calculated molecular weight
83,736 MW
Background of RSK2
Ribosomal protein S6 kinase, 90kDa, polypeptide 3, also known as RPS6KA3, is an enzyme that in humans is encoded by the RPS6KA3 gene. It is mapped to Xp22.12. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02215-2 is guaranteed for ELISA, Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human CACO-2 whole cell lysates, human SH-SY5Y whole cell lysates, human 293T whole cell lysates, human A549 whole cell lysates, rat skeletal muscle tissue lysates, rat thymus tissue lysates, mouse skeletal muscle tissue lysates, mouse thymus tissue lysates
ICC/IF: SiHa cell
IP: A549 cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat thymus tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates,
Lane 8: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (Catalog # A02215-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6KA3 at approximately 84 kDa. The expected band size for RPS6KA3 is at 84 kDa.
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Figure 2. IF analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2) and anti-Beta Tubulin antibody (M01857-3).
RPS6KA3 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS6KA3 Antibody (A02215-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Immunoprecipitating RPS6KA3 in A549 whole cell lysate .
Western blot analysis of RPS6KA3 using anti-RPS6KA3 antibody (A02215-2).
Lane 1: A549 whole cell lysates (30ug)
Lane 2: Rabbit control IgG instead of anti-RPS6KA3 antibody in A549 whole cell lysate.
Lane 3: anti-RPS6KA3 antibody (2μg) + A549 whole cell lysate (500μg)
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPS6KA3 antigen affinity purified polyclonal antibody (A02215-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RPS6KA3 at approximately 84 kDa. The expected band size for RPS6KA3 is at 84 kDa.
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Figure 4. Flow Cytometry analysis of 293T cells using anti-RPS6KA3 antibody (A02215-2).
Overlay histogram showing 293T cells stained with A02215-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS6KA3 Antibody (A02215-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RPS6KA3 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA3
Full Name
Ribosomal protein S6 kinase alpha-3
Weight
83,736 MW
Superfamily
protein kinase superfamily
Alternative Names
Ribosomal protein S6 kinase alpha-3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 3; p90-RSK 3; P90rsk3; Insulin-stimulated protein kinase 1; ISPK-1; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; Ribosomal S6 kinase 2; RSK-2; pp90RSK2; RPS6KA3; ISPK1; MAPKAPK1B; RSK2 RPS6KA3 CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2 ribosomal protein S6 kinase A3 ribosomal protein S6 kinase alpha-3|MAP kinase-activated protein kinase 1b|MAPK-activated protein kinase 1b|MAPKAP kinase 1b|MAPKAPK-1b|RSK-2|S6K-alpha-3|epididymis secretory sperm binding protein|insulin-stimulated protein kinase 1|p90-RSK 3|ribosomal S6 kinase 2|ribosomal protein S6 kinase, 90kDa, polypeptide 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KA3, check out the RPS6KA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rsk 2/MAPKAP Kinase 1b/RPS6KA3 Picoband® Antibody (A02215-2)
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