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Product Info Summary
| SKU: | A05439-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RRN3 Antibody Picoband®
SKU/Catalog Number
A05439-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RRN3 Antibody Picoband® catalog # A05439-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RRN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05439-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human RRN3 recombinant protein (Position: D184-L651). Human RRN3 shares 87.4% amino acid (aa) sequence identity with mouse RRN3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05439-2 is reactive to RRN3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
74 kDa
Calculated molecular weight
74 kDa
Background of RRN3
RNA polymerase I-specific transcription initiation factor RRN3 is an enzyme that in humans is encoded by the RRN3 gene. This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05439-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human SH-SY5Y whole cell, human HepG2 whole cell, human MCF-7 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RRN3 using anti-RRN3 antibody (A05439-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRN3 antigen affinity purified polyclonal antibody (Catalog # A05439-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRN3 at approximately 74 kDa. The expected band size for RRN3 is at 74 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-RRN3 antibody (A05439-2).
Overlay histogram showing HepG2 cells stained with A05439-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRN3 Antibody (A05439-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RRN3 (Source: Uniprot.org, NCBI)
Gene Name
RRN3
Full Name
RNA polymerase I-specific transcription initiation factor RRN3
Weight
74 kDa
Superfamily
RRN3 family
Alternative Names
DKFZp566E104; RNA polymerase I-specific transcription initiation factor RRN3; RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae); RRN3 RNA polymerase I transcription factor homolog (yeast); TIF-IA; TIFIAMGC104238; Transcription initiation factor IA; transcription initiation factor TIF-IA RRN3 A-270G1.2, TIFIA RRN3 homolog, RNA polymerase I transcription factor RNA polymerase I-specific transcription initiation factor RRN3|RRN3 RNA polymerase I transcription factor homolog|TIF-IA|transcription initiation factor IA|transcription initiation factor TIF-IA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRN3, check out the RRN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RRN3 Antibody Picoband® (A05439-2)
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