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Product Info Summary
| SKU: | M01978 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-RRM2 Monoclonal Antibody
SKU/Catalog Number
M01978
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RRM2 Monoclonal Antibody catalog # M01978. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RRM2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01978)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEAE-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RRM2 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01978 is reactive to RRM2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
18473 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01978 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human SIHA whole cell, human U251 whole cell, human MCF-7 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RRM2 using anti-RRM2 antibody (M01978).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM2 antigen affinity purified monoclonal antibody (Catalog # M01978) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRM2 at approximately 45 kDa. The expected band size for RRM2 is at 45 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RRM2 (Source: Uniprot.org, NCBI)
Gene Name
RRM2
Full Name
Ribonucleoside-diphosphate reductase subunit M2
Weight
18473 MW
Superfamily
ribonucleoside diphosphate reductase small chain family
Alternative Names
Ubiquitin D;Diubiquitin;Ubiquitin-like protein FAT10;UBD;FAT10; RRM2 C2orf48, R2, RR2, RR2M ribonucleotide reductase regulatory subunit M2 ribonucleoside-diphosphate reductase subunit M2|ribonucleotide reductase M2 polypeptide|ribonucleotide reductase small chain|ribonucleotide reductase small subunit|uncharacterized protein C2orf48
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRM2, check out the RRM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RRM2 Monoclonal Antibody (M01978)
Hello CJ!
M01978 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu Lx, Li Zh, Tao Yf, Li Rh, Fang F, Zhao H, Li G, Li Yh, Wang J, Feng X, Pan J. J Exp Clin Cancer Res. 2014 Dec 19;33:108. Doi: 10.1186/S13046-014-0108-3. Histone Acetyltransferase Inhibitor Ii Induces Apoptosis In Glioma Cell Lines Via The P53 S...
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Customer Q&As
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5 Customer Q&As for Anti-RRM2 Monoclonal Antibody
Question
We have been able to see staining in human neonatal skin. Are there any suggestions? Is anti-RRM2 Monoclonal antibody supposed to stain neonatal skin positively?
W. Patel
Verified customer
Asked: 2020-01-10
Answer
Based on literature neonatal skin does express RRM2. Based on Uniprot.org, RRM2 is expressed in secondary oocyte, mammary carcinoma, neonatal skin, muscle skin, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have RRM2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Mammary carcinoma, Pubmed ID: 1627826
Muscle, and Skin, Pubmed ID: 15489334
Neonatal skin, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-10
Question
What sample was tested in WB and IHC applications with M01978?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of RRM2 expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We purchased anti-RRM2 Monoclonal antibody for IP on secondary oocyte a few years ago. I am using human, and We are going to use the antibody for IF next. I am interested in examining secondary oocyte as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
I have checked the website and datasheets of our anti-RRM2 Monoclonal antibody and it appears that M01978 has been validated on human in both IP and IF. Thus M01978 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-27
Question
We were satisfied with the WB result of your anti-RRM2 Monoclonal antibody. However we have been able to see positive staining in secondary oocyte cytoplasm. using this antibody. Is that expected? Could you tell me where is RRM2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
From literature, secondary oocyte does express RRM2. Generally RRM2 expresses in cytoplasm. Regarding which tissues have RRM2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Mammary carcinoma, Pubmed ID: 1627826
Muscle, and Skin, Pubmed ID: 15489334
Neonatal skin, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-26
Question
We are currently using anti-RRM2 Monoclonal antibody M01978 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-06
Answer
The anti-RRM2 Monoclonal antibody (M01978) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-06
