Click image to see more details
Product Info Summary
| SKU: | M07839 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RPS8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M07839
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-RPS8 Rabbit Monoclonal Antibody catalog # M07839. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPS8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07839)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
30R72
Isotype
IgG
Immunogen
A synthesized peptide derived from human RPS8
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07839 is reactive to RPS8 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
26 kDa
Calculated molecular weight
25279 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07839 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human 293T whole cell, human THP-1 whole cell, human K562 whole cell, human HEL whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse ANA-1 whole cell, mouse RAW264.7 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RPS8 using anti-RPS8 antibody (M07839).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse ANA-1 whole cell lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS8 antigen affinity purified monoclonal antibody (M07839) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS8 at approximately 26 kDa. The expected band size for RPS8 is at 24 kDa.
Click image to see more details
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
Protein Target Info & Infographic
Gene/Protein Information For RPS8 (Source: Uniprot.org, NCBI)
Gene Name
RPS8
Full Name
40S ribosomal protein S8
Weight
25279 MW
Superfamily
eukaryotic ribosomal protein eS8 family
Alternative Names
Ubiquitin-like-conjugating enzyme ATG10;6.3.2.-;Autophagy-related protein 10;APG10-like;ATG10;APG10L;PP12616; RPS8 S8 ribosomal protein S8 40S ribosomal protein S8|OK/SW-cl.83|small ribosomal subunit protein eS8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS8, check out the RPS8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPS8 Rabbit Monoclonal Antibody (M07839)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RPS8 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-RPS8 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
