Product Info Summary
SKU: | M08246-1 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-RPL7A Rabbit Monoclonal Antibody
SKU/Catalog Number
M08246-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-RPL7A Rabbit Monoclonal Antibody catalog # M08246-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPL7A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08246-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
25R39
Isotype
IgG
Immunogen
A synthesized peptide derived from human RPL7A
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08246-1 is reactive to RPL7A in Human, Mouse, Rat
Applications
M08246-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
30 kDa
Calculated molecular weight
16383 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
Validation Images & Assay Conditions
![m08246 1 rpl7a primary antibodies wb testing 1 m08246 1 rpl7a primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/m/0/m08246-1-rpl7a-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of RPL7A using anti-RPL7A antibody (M08246-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse pancreas tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL7A antigen affinity purified monoclonal antibody (Catalog # M08246-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL7A at approximately 30 kDa. The expected band size for RPL7A is at 30 kDa.
![m08246 1 rpl7a primary antibodies ihc testing 2 m08246 1 rpl7a primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/m/0/m08246-1-rpl7a-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of RPL7A using anti-RPL7A antibody (M08246-1).
RPL7A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPL7A Antibody (M08246-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![m08246 1 rpl7a primary antibodies ihc testing 3 m08246 1 rpl7a primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/m/0/m08246-1-rpl7a-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of RPL7A using anti-RPL7A antibody (M08246-1).
RPL7A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPL7A Antibody (M08246-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![m08246 1 rpl7a primary antibodies ihc testing 4 m08246 1 rpl7a primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/m/0/m08246-1-rpl7a-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of RPL7A using anti-RPL7A antibody (M08246-1).
RPL7A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RPL7A Antibody (M08246-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RPL7A (Source: Uniprot.org, NCBI)
Gene Name
RPL7A
Full Name
60S ribosomal protein L7a
Weight
16383 MW
Superfamily
eukaryotic ribosomal protein eL8 family
Alternative Names
60S ribosomal protein L7a; PLA-X polypeptide; ribosomal protein L7a; SURF-3; SURF3surfeit 3; Surfeit locus protein 3; thyroid hormone receptor uncoupling protein; TRUP RPL7A L7A, SURF3, TRUP ribosomal protein L7a 60S ribosomal protein L7a|PLA-X polypeptide|large ribosomal subunit protein eL8|surfeit 3|surfeit locus protein 3|thyroid hormone receptor uncoupling protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL7A, check out the RPL7A Infographic
![RPL7A infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL7A Rabbit Monoclonal Antibody (M08246-1)
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