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Product Info Summary
| SKU: | PB10092 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-RPL19 Antibody Picoband®
SKU/Catalog Number
PB10092
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RPL19 Antibody Picoband® catalog # PB10092. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPL19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10092)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RPL19, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10092 is reactive to RPL19 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
23466 MW
Background of RPL19
60S ribosomal protein L19 is a protein that in humans is encoded by the RPL19 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L19E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10092 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human MCF-7 whole cell, human A2780 whole cell, human 293T whole cell, human Jurkat whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
ICC/IF: U20S cell
FCM: U20S cell, A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RPL19 using anti-RPL19 antibody (PB10092).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A2780 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL19 antigen affinity purified polyclonal antibody (Catalog # PB10092) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL19 at approximately 26 kDa. The expected band size for RPL19 is at 23 kDa.
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Figure 2. IF analysis of RPL19 using anti-RPL19 antibody (PB10092).
RPL19 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RPL19 Antibody (PB10092) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of U20S cells using anti-RPL19 antibody (PB10092).
Overlay histogram showing U20S cells stained with PB10092 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL19 Antibody (PB10092,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. Flow Cytometry analysis of A431 cells using anti-RPL19 antibody (PB10092).
Overlay histogram showing A431 cells stained with PB10092 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL19 Antibody (PB10092,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RPL19 (Source: Uniprot.org, NCBI)
Gene Name
RPL19
Full Name
60S ribosomal protein L19
Weight
23466 MW
Superfamily
eukaryotic ribosomal protein eL19 family
Alternative Names
60S ribosomal protein L19;RPL19; RPL19 L19 ribosomal protein L19 60S ribosomal protein L19|large ribosomal subunit protein eL19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL19, check out the RPL19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL19 Antibody Picoband® (PB10092)
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Customer Q&As
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7 Customer Q&As for Anti-RPL19 Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for brain using anti-RPL19 antibody PB10092. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-31
Question
We are currently using anti-RPL19 antibody PB10092 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-RPL19 antibody (PB10092) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-RPL19 antibody PB10092. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-14
Question
Will anti-RPL19 antibody PB10092 work for IF with brain?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
According to the expression profile of brain, RPL19 is highly expressed in brain. So, it is likely that anti-RPL19 antibody PB10092 will work for IF with brain.
Boster Scientific Support
Answered: 2019-01-30
Question
Is a blocking peptide available for product anti-RPL19 antibody (PB10092)?
Verified Customer
Verified customer
Asked: 2017-08-10
Answer
We do provide the blocking peptide for product anti-RPL19 antibody (PB10092). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-10
Question
Would PB10092 anti-RPL19 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Brown
Verified customer
Asked: 2015-07-24
Answer
You can see on the product datasheet, PB10092 anti-RPL19 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-07-24
Question
We need to test anti-RPL19 antibody PB10092 on rat brain for research purposes, then I may be interested in using anti-RPL19 antibody PB10092 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Lewis
Verified customer
Asked: 2013-03-14
Answer
The products we sell, including anti-RPL19 antibody PB10092, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-14
