Product Info Summary
SKU: | A07136 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-RPL15/Ribosomal Protein L15 Antibody
SKU/Catalog Number
A07136
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RPL15/Ribosomal Protein L15 Antibody (Catalog # A07136). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPL15/Ribosomal Protein L15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07136)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPL15.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07136 is reactive to RPL15 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
24146 MW
Background of RPL15
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Alternate splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Herzog H.; Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
Wang Q., Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Yoshihama M., Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07136 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells and LOVO cells, using RPL15 antibody A07136.
Protein Target Info & Infographic
Gene/Protein Information For RPL15 (Source: Uniprot.org, NCBI)
Gene Name
RPL15
Full Name
60S ribosomal protein L15
Weight
24146 MW
Superfamily
eukaryotic ribosomal protein eL15 family
Alternative Names
60S ribosomal protein L15;RPL15;EC45;TCBAP0781; RPL15 DBA12, EC45, L15, RPL10, RPLY10, RPYL10 ribosomal protein L15 60S ribosomal protein L15|large ribosomal subunit protein eL15
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL15, check out the RPL15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL15/Ribosomal Protein L15 Antibody (A07136)
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Customer Q&As
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4 Customer Q&As for Anti-RPL15/Ribosomal Protein L15 Antibody
Question
Does A07136 anti-RPL15/Ribosomal Protein L15 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
As indicated on the product datasheet, A07136 anti-RPL15/Ribosomal Protein L15 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-06
Question
Do you have a BSA free version of anti-RPL15/Ribosomal Protein L15 antibody A07136 available?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RPL15/Ribosomal Protein L15 antibody A07136 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-19
Question
I see that the anti-RPL15/Ribosomal Protein L15 antibody A07136 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-26
Question
We are currently using anti-RPL15/Ribosomal Protein L15 antibody A07136 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
The anti-RPL15/Ribosomal Protein L15 antibody (A07136) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-17