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Product Info Summary
| SKU: | A06941-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MAL Antibody Picoband®
SKU/Catalog Number
A06941-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAL Antibody Picoband® catalog # A06941-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06941-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MAL, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06941-1 is reactive to MAL in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
87148 MW
Background of MAL
Myelin and lymphocyte protein is a protein that in humans is encoded by the MAL gene. The protein encoded by this gene is a highly hydrophobic integral membrane protein belonging to the MAL family of proteolipids. The protein has been localized to the endoplasmic reticulum of T-cells and is a candidate linker protein in T-cell signal transduction. In addition, this proteolipid is localized in compact myelin of cells in the nervous system and has been implicated in myelin biogenesis and/or function. The protein plays a role in the formation, stabilization and maintenance of glycosphingolipid-enriched membrane microdomains. Down-regulation of this gene has been associated with a variety of human epithelial malignancies. Alternative splicing produces four transcript variants which vary from each other by the presence or absence of alternatively spliced exons 2 and 3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06941-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Positive Control
WB: human Jurkat whole cell, human Raji whole cell, human HL-60 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAL using anti-MAL antibody (A06941-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human HL-60 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAL antigen affinity purified polyclonal antibody (Catalog # A06941-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAL at approximately 17KD. The expected band size for MAL is at 17KD.
Protein Target Info & Infographic
Gene/Protein Information For MAL (Source: Uniprot.org, NCBI)
Gene Name
MAL
Full Name
Myelin and lymphocyte protein
Weight
87148 MW
Superfamily
MAL family
Alternative Names
Semaphorin-3E; SEMA3E; KIAA0331; SEMAH MAL MVP17, VIP17 mal, T cell differentiation protein myelin and lymphocyte protein|T-lymphocyte maturation-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAL, check out the MAL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAL Antibody Picoband® (A06941-1)
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