Product Info Summary
SKU: | A07781 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-RPL14/Ribosomal Protein L14 Antibody
SKU/Catalog Number
A07781
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RPL14/Ribosomal Protein L14 Antibody (Catalog # A07781). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPL14/Ribosomal Protein L14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07781)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPL14.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07781 is reactive to RPL14 in Human
Reconstitution
Calculated molecular weight
23432 MW
Background of RPL14
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Aoki M., Diabetes 45:157-164(1996).
Tanaka M., Biochem. Biophys. Res. Commun. 243:531-537(1998).
Yoshihama M., Genome Res. 12:379-390(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07781 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, using RPL14 antibody A07781.
Protein Target Info & Infographic
Gene/Protein Information For RPL14 (Source: Uniprot.org, NCBI)
Gene Name
RPL14
Full Name
60S ribosomal protein L14
Weight
23432 MW
Superfamily
eukaryotic ribosomal protein eL14 family
Alternative Names
60S ribosomal protein L14;CAG-ISL 7;RPL14; RPL14 CAG-ISL-7, CTG-B33, L14, RL14, hRL14 ribosomal protein L14 60S ribosomal protein L14|large ribosomal subunit protein eL14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL14, check out the RPL14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL14/Ribosomal Protein L14 Antibody (A07781)
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No publications found for A07781
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Customer Q&As
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5 Customer Q&As for Anti-RPL14/Ribosomal Protein L14 Antibody
Question
Does anti-RPL14/Ribosomal Protein L14 antibody A07781 work on monkey WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
Our lab technicians have not tested anti-RPL14/Ribosomal Protein L14 antibody A07781 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-RPL14/Ribosomal Protein L14 antibody A07781 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-08
Question
We are currently using anti-RPL14/Ribosomal Protein L14 antibody A07781 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The anti-RPL14/Ribosomal Protein L14 antibody (A07781) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-25
Question
Is this A07781 anti-RPL14/Ribosomal Protein L14 antibody reactive to the isotypes of RPL14?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The immunogen of A07781 anti-RPL14/Ribosomal Protein L14 antibody is Synthesized peptide derived from internal of human RPL14. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-23
Question
Can you help my question with product A07781, anti-RPL14/Ribosomal Protein L14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07781 anti-RPL14/Ribosomal Protein L14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-09
Question
I would like to test anti-RPL14/Ribosomal Protein L14 antibody A07781 on human umbilical vein for research purposes, then I may be interested in using anti-RPL14/Ribosomal Protein L14 antibody A07781 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
The products we sell, including anti-RPL14/Ribosomal Protein L14 antibody A07781, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-20