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Product Info Summary
| SKU: | A04479-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ROBO4 Antibody Picoband®
SKU/Catalog Number
A04479-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-ROBO4 Antibody Picoband® catalog # A04479-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-ROBO4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04479-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ROBO4 recombinant protein (Position: D108-A933).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04479-2 is reactive to ROBO4 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
107 kDa
Calculated molecular weight
128770 MW
Background of ROBO4
Roundabout homolog 4 is a protein that in humans is encoded by the ROBO4 gene. Predicted to enable cell-cell adhesion mediator activity. Involved in angiogenesis and establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04479-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, rat PC-12 whole cell, mouse LLC whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ROBO4 using anti-ROBO4 antibody (A04479-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse LLC whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROBO4 antigen affinity purified polyclonal antibody (Catalog # A04479-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROBO4 at approximately 107 kDa. The expected band size for ROBO4 is at 90-170 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ROBO4 (Source: Uniprot.org, NCBI)
Gene Name
ROBO4
Full Name
Roundabout homolog 4
Weight
128770 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Integrin alpha-L;CD11 antigen-like family member A;Leukocyte adhesion glycoprotein LFA-1 alpha chain;LFA-1A;Leukocyte function-associated molecule 1 alpha chain;CD11a;ITGAL;CD11A; ROBO4 AOVD3, ECSM4, MRB roundabout guidance receptor 4 roundabout homolog 4|roundabout homolog 4, magic roundabout|roundabout, axon guidance receptor, homolog 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ROBO4, check out the ROBO4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ROBO4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ROBO4 Antibody Picoband® (A04479-2)
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