Click image to see more details
Product Info Summary
| SKU: | A00707 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RNF8 Antibody
SKU/Catalog Number
A00707
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RNF8 Antibody (Catalog # A00707). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RNF8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RNF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00707)
Host
Rabbit
Contents
RNF8 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8. The immunogen is located within amino acids 330 - 380 of RNF8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of RNF8 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A00707 is reactive to RNF8 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
55518 MW
Background of RNF8
RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00707 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 μg/mL.
Click image to see more details
Immunofluorescence of RNF8 in Rat Lung tissue with RNF8 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RNF8 (Source: Uniprot.org, NCBI)
Gene Name
RNF8
Full Name
E3 ubiquitin-protein ligase RNF8
Weight
55518 MW
Superfamily
RNF8 family
Alternative Names
hRNF8, KIAA0646, E3 ubiquitin-protein ligase RNF8, RING finger protein 8, hRNF8 RNF8 hRNF8 ring finger protein 8 E3 ubiquitin-protein ligase RNF8|C3HC4-type zinc finger protein|RING-type E3 ubiquitin transferase RNF8|UBC13/UEV-interacting ring finger protein|ring finger protein (C3HC4 type) 8|ring finger protein 8, E3 ubiquitin protein ligase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF8, check out the RNF8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF8 Antibody (A00707)
Hello CJ!
No publications found for A00707
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RNF8 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-RNF8 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
