Anti-RNF8 Antibody

Product Name

Anti-RNF8 Antibody

View all RNF8 Antibodies

SKU/Catalog Number

A00707

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RNF8 Antibody (Catalog # A00707). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RNF8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RNF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00707)

Host

Rabbit

Contents

RNF8 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8. The immunogen is located within amino acids 330 - 380 of RNF8.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of RNF8 are known to exist; this antibody will detect all three isoforms.

Reactive Species

A00707 is reactive to RNF8 in Human, Mouse, Rat

Applications

A00707 is guaranteed for ELISA, IF, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

55518 MW

Background of RNF8

RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) 1 and (B) 2 μg/mL.

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Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 μg/mL.

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Immunofluorescence of RNF8 in Rat Lung tissue with RNF8 antibody at 20 μg/mL.

Gene/Protein Information For RNF8 (Source: Uniprot.org, NCBI)

Alternative Names

ActA Binding Protein 2; C3HC4-type zinc finger protein; EC 6.3.2; EC 6.3.2.-; FLJ12013; KIAA0646UBC13/UEV-interacting ring finger protein; ring finger protein (C3HC4 type) 8; ring finger protein 8E3 ubiquitin-protein ligase RNF8; RNF8 RNF8 hRNF8 ring finger protein 8 E3 ubiquitin-protein ligase RNF8|C3HC4-type zinc finger protein|RING-type E3 ubiquitin transferase RNF8|UBC13/UEV-interacting ring finger protein|ring finger protein (C3HC4 type) 8|ring finger protein 8, E3 ubiquitin protein ligase

For more info on RNF8, check out the RNF8 Infographic

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In this infographic, you will see the following information for RNF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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