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Product Info Summary
| SKU: | A04457-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RNF31/HOIP Antibody Picoband®
View all HOIP/RNF31 Antibodies
SKU/Catalog Number
A04457-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RNF31/HOIP Antibody Picoband® catalog # A04457-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF31/HOIP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04457-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RNF31/HOIP recombinant protein (Position: R970-K1072).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04457-3 is reactive to RNF31 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
119.652kDa
Background of HOIP/RNF31
RING finger protein 31 is a protein that in humans is encoded by the RNF31 gene. It is mapped to 14q12. The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. The encoded protein is the E3 ubiquitin-protein ligase component of the linear ubiquitin chain assembly complex. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04457-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissues, human HepG2 whole cell, human PC-3 whole cell, human A549 whole cell, human U2OS whole cell, human Caco-2 whole cell, human Hela whole cell,
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RNF31 using anti-RNF31 antibody (A04457-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissues lysates
Lane 2: human HepG2 whole cell lysates
Lane 3: human PC-3 whole cell lysates
Lane 4: human A549 whole cell lysates
Lane 5: human U2OS whole cell lysates
Lane 6: human Caco-2 whole cell lysates
Lane 7: human Hela whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF31 antigen affinity purified polyclonal antibody (Catalog # A04457-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF31 at approximately 120KD. The expected band size for RNF31 is at 120KD.
Protein Target Info & Infographic
Gene/Protein Information For RNF31 (Source: Uniprot.org, NCBI)
Gene Name
RNF31
Full Name
E3 ubiquitin-protein ligase RNF31
Weight
119.652kDa
Superfamily
RBR family
Alternative Names
E3 ubiquitin-protein ligase RNF31; HOIL-1-interacting protein; HOIP; RING finger protein 31; RING-type E3 ubiquitin transferase RNF31; Zinc in-between-RING-finger ubiquitin-associated domain protein; RNF31; ZIBRA RNF31 HOIP, Paul, ZIBRA ring finger protein 31 E3 ubiquitin-protein ligase RNF31|HOIL-1-interacting protein|RING-type E3 ubiquitin transferase RNF31|zinc in-between-RING-finger ubiquitin-associated domain protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF31, check out the RNF31 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF31: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF31/HOIP Antibody Picoband® (A04457-3)
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No publications found for A04457-3
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Customer Q&As
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13 Customer Q&As for Anti-RNF31/HOIP Antibody Picoband®
Question
Is a blocking peptide available for product anti-RNF31/HOIP antibody (A04457-3)?
J. Li
Verified customer
Asked: 2020-04-09
Answer
We do provide the blocking peptide for product anti-RNF31/HOIP antibody (A04457-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-09
Question
Will A04457-3 anti-RNF31/HOIP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
It shows on the product datasheet, A04457-3 anti-RNF31/HOIP antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-07
Question
Do you have a BSA free version of anti-RNF31/HOIP antibody A04457-3 available?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RNF31/HOIP antibody A04457-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-10
Question
Please see the WB image, lot number and protocol we used for mammary carcinoma using anti-RNF31/HOIP antibody A04457-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
Is this A04457-3 anti-RNF31/HOIP antibody reactive to the isotypes of RNF31?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The immunogen of A04457-3 anti-RNF31/HOIP antibody is E.coli-derived human RNF31/HOIP recombinant protein (Position: R970-K1072). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-20
Question
We are interested in to test anti-RNF31/HOIP antibody A04457-3 on rat mammary carcinoma for research purposes, then I may be interested in using anti-RNF31/HOIP antibody A04457-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The products we sell, including anti-RNF31/HOIP antibody A04457-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-08
Question
I have a question about product A04457-3, anti-RNF31/HOIP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04457-3 anti-RNF31/HOIP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-03
Question
I was wanting to use your anti-RNF31/HOIP antibody for ELISA for rat mammary carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat mammary carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
It shows on the product datasheet, A04457-3 anti-RNF31/HOIP antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat mammary carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
I see that the anti-RNF31/HOIP antibody A04457-3 works with ELISA, what is the protocol used to produce the result images on the product page?
K. Taylor
Verified customer
Asked: 2019-07-03
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-03
Question
We are currently using anti-RNF31/HOIP antibody A04457-3 for mouse tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
The anti-RNF31/HOIP antibody (A04457-3) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-21
Question
Will anti-RNF31/HOIP antibody A04457-3 work on bovine ELISA with spleen?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
Our lab technicians have not validated anti-RNF31/HOIP antibody A04457-3 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-RNF31/HOIP antibody A04457-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine spleen in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-04
Question
Does anti-RNF31/HOIP antibody A04457-3 work for ELISA with mammary carcinoma?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
According to the expression profile of mammary carcinoma, RNF31 is highly expressed in mammary carcinoma. So, it is likely that anti-RNF31/HOIP antibody A04457-3 will work for ELISA with mammary carcinoma.
Boster Scientific Support
Answered: 2018-02-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary carcinoma using anti-RNF31/HOIP antibody A04457-3. Let me know if you need anything else.
P. Carter
Verified customer
Asked: 2014-01-02
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-02
