Anti-RNF168 Antibody

Product Name

Anti-RNF168 Antibody

View all Rnf168 Antibodies

SKU/Catalog Number

A01224

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RNF168 Antibody (Catalog # A01224). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

RNF168 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RNF168 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01224)

Host

Rabbit

Contents

RNF168 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RNF168 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human RNF168. The immunogen is located within amino acids 440 - 490 of RNF168.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.

Reactive Species

A01224 is reactive to Rnf168 in Human, Mouse

Applications

A01224 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

65020 MW

Background of Rnf168

RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RNF168 antibody can be used for detection of RNF168 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of RNF168 in human brain tissue lysate with RNF168 antibody at 1 μg/mL.

Gene/Protein Information For Rnf168 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase RNF168; EC 6.3.2; EC 6.3.2.-; FLJ39749; ring finger protein 168FLJ35794; RNF168 Rnf168|3110001H15Rik|ring finger protein 168|E3 ubiquitin-protein ligase RNF168|RING-type E3 ubiquitin transferase RNF168|ring fnger protein 168

For more info on Rnf168, check out the Rnf168 Infographic

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In this infographic, you will see the following information for Rnf168: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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