Anti-RKHD3 MEX3B Antibody

Product Name

Anti-RKHD3 MEX3B Antibody

View all RKHD3 Antibodies

SKU/Catalog Number

A10435

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-RKHD3 MEX3B Antibody (Catalog # A10435). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

RKHD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-RKHD3 MEX3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10435)

Host

Rabbit

Contents

RKHD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RKHD3 antibody was raised against a 16 amino acid synthetic peptide from near the center of human RKHD3. The immunogen is located within amino acids 180 - 230 of RKHD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody is specific for RKHD3 and will not recognize the other RKHD3 family of proteins.

Reactive Species

A10435 is reactive to MEX3B in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

58832 MW

Background of RKHD3

Rkhd3, also known as MEX3B is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd3, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd3 is thought to act as a mechanism to fine-tune mRNA regulation in early Xenopus development, and with Rkhd4, but not Rkhd1, will co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10435 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RKHD3 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For MEX3B (Source: Uniprot.org, NCBI)

Alternative Names

RKHD3, MEX-3B, RNF195, KIAA2009, RKHD3, RNA-binding protein MEX3B, RING finger and KH domain-containing protein 3 MEX3B MEX-3B, RKHD3, RNF195 mex-3 RNA binding family member B RNA-binding protein MEX3B|RING finger protein 195|ring finger and KH domain containing 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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