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Product Info Summary
| SKU: | A16655-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RGL4 Antibody Picoband®
SKU/Catalog Number
A16655-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RGL4 Antibody Picoband® catalog # A16655-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RGL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16655-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RGL4 recombinant protein (Position: R37-R381).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A16655-1 is reactive to RGL4 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
24145 MW
Background of RGL4
This oncogene encodes a protein similar to guanine nucleotide exchange factor Ral guanine dissociation stimulator. Increased expression of this gene leads to translocation of the encoded protein to the cell membrane. The encoded protein can activate several pathways, including the Ras-Raf-MEK-ERK cascade.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16655-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MOLT-4 whole cell, human 293T whole cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RGL4 using anti-RGL4 antibody (A16655-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGL4 antigen affinity purified polyclonal antibody (Catalog # A16655-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGL4 at approximately 52 kDa. The expected band size for RGL4 is at 52 kDa.
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Figure 2. Flow Cytometry analysis of SiHa cells using anti-RGL4 antibody (A16655-1).
Overlay histogram showing SiHa cells stained with A16655-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGL4 Antibody (A16655-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RGL4 (Source: Uniprot.org, NCBI)
Gene Name
RGL4
Full Name
Ral-GDS-related protein
Weight
24145 MW
Alternative Names
Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489 RGL4 Rgr ral guanine nucleotide dissociation stimulator like 4 ral-GDS-related protein|Ral-GDS related protein Rgr|RalGDS related oncogene|ralGDS-like 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RGL4, check out the RGL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RGL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RGL4 Antibody Picoband® (A16655-1)
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