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Product Info Summary
| SKU: | A07674 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Transcription factor RFX3 RFX3 Antibody
SKU/Catalog Number
A07674
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Transcription factor RFX3 RFX3 Antibody catalog # A07674. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor RFX3 RFX3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07674)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RFX3. AA range:641-690
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07674 is reactive to RFX3 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
83530 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07674 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using RFX3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For RFX3 (Source: Uniprot.org, NCBI)
Gene Name
RFX3
Full Name
Transcription factor RFX3
Weight
83530 MW
Superfamily
RFX family
Alternative Names
Transcription factor RFX3;Regulatory factor X 3;RFX3; RFX3 regulatory factor X3 transcription factor RFX3|DNA binding protein RFX3|regulatory factor X, 3 (influences HLA class II expression)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RFX3, check out the RFX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RFX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor RFX3 RFX3 Antibody (A07674)
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No publications found for A07674
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Transcription factor RFX3 RFX3 Antibody
Question
We are currently using anti-RFX3 antibody A07674 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The anti-RFX3 antibody (A07674) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-11
Question
Is a blocking peptide available for product anti-RFX3 antibody (A07674)?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We do provide the blocking peptide for product anti-RFX3 antibody (A07674). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-20
Question
My question regarding product A07674, anti-RFX3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07674 anti-RFX3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-23
Question
Will anti-RFX3 antibody A07674 work for WB with muscle testis?
J. Anderson
Verified customer
Asked: 2018-05-03
Answer
According to the expression profile of muscle testis, RFX3 is highly expressed in muscle testis. So, it is likely that anti-RFX3 antibody A07674 will work for WB with muscle testis.
Boster Scientific Support
Answered: 2018-05-03
Question
I was wanting to use your anti-RFX3 antibody for WB for human muscle testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human muscle testis identification?
K. Kulkarni
Verified customer
Asked: 2017-10-20
Answer
It shows on the product datasheet, A07674 anti-RFX3 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human muscle testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-20
Question
Does anti-RFX3 antibody A07674 work on goat WB with cerebellum?
B. Evans
Verified customer
Asked: 2017-06-05
Answer
Our lab technicians have not validated anti-RFX3 antibody A07674 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RFX3 antibody A07674 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-05
Question
Would A07674 anti-RFX3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Johnson
Verified customer
Asked: 2013-06-07
Answer
You can see on the product datasheet, A07674 anti-RFX3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-07
