Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody

Product Name

Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody

View all RBP Antibodies

SKU/Catalog Number

M03820

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody (Catalog # M03820). Tested in IHC applications. This antibody reacts with Human, Chimpanzee, Monkey, Goat, Rabbit, Rat, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Retinol Binding Protein-1 (RBP1) RBP4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03820)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: G4E4

Isotype

IgG1, kappa

Immunogen

Retinol binding protein-1 Purified from human plasma

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03820 is reactive to RBP in Goat, Human, Monkey, Mouse, Rabbit, Rat, Chimpanzee

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

23010 MW

Background of RBP

This monoclonal antibody recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03820 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For RBP (Source: Uniprot.org, NCBI)

Alternative Names

Retinol-binding protein 4;Plasma retinol-binding protein;PRBP;RBP;Plasma retinol-binding protein (1-182);Plasma retinol-binding protein (1-181);Plasma retinol-binding protein (1-179);Plasma retinol-binding protein (1-176);RBP4;PRO2222;

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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