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Product Info Summary
| SKU: | EK0832 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®
View all RBP4/Retinol-Binding Protein 4 ELISA kits
SKU/Catalog Number
EK0832
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Rbp4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0832)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E19-L201
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0832 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0832 is reactive to Rbp4 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0832 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of RBP4/Retinol-Binding Protein 4
Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37—K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0832-CAP | Anti-Mouse Rbp4 Pre-coated 96-well strip microplate | 1 |
| EK0832-ST | Mouse Rbp4 Standard | 2 vials, 20 ng/tube |
| EK0832-DA | Mouse Rbp4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.090 | 0.141 | 0.192 | 0.300 | 0.521 | 0.939 | 1.677 | 2.337 |
Data Example Images
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Mouse RBP4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect RBP4/Retinol-Binding Protein 4, Dilution ratio of 1:500, concentration in serum and plasma is 1-2 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 418 | 2343 | 8435 | 401 | 2576 | 7635 |
| Standard deviation | 23.4 | 105.43 | 649.49 | 28.07 | 159.71 | 633.7 |
| CV (%) | 4.5% | 4.5% | 7.7% | 7% | 6.2% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of RBP4/Retinol-Binding Protein 4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 418 | 421 | 430 | 389 | 414 | 15.37 | 3.7% |
| Sample 2 | 2343 | 2347 | 2512 | 2667 | 2467 | 133.97 | 5.4% |
| Sample 3 | 8435 | 8668 | 9897 | 9494 | 9123 | 595.2 | 6.5% |
Protein Target Info & Infographic
Gene/Protein Information For Rbp4 (Source: Uniprot.Org, NCBI)
Gene Name
Rbp4
Full Name
Retinol-binding protein 4
Weight
28405 MW
Superfamily
calycin superfamily
Alternative Names
Retinol-binding protein 4 ;Rbp4 ; RBP4 MCOPCB10, RDCCAS retinol binding protein 4 retinol-binding protein 4|PRBP|RBP|plasma retinol-binding protein|retinol binding protein 4, plasma|retinol-binding protein 4, interstitial
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Rbp4, check out the Rbp4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Rbp4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine® (EK0832)
Hello CJ!
EK0832 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Diabetes-induced upregulation of urotensin II and its receptor plays an important role in TGF-β1-mediated renal fibrosis and dysfunction
Proteomics analysis in the kidney of mice following oral feeding Realgar
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis RBP4 using the EK0832 Mouse RBP4 Picokine® ELISA Kit?
C. Turner
Verified customer
Asked: 2020-05-15
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-05-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-25
Question
Q: how to analyze ELISA data? I have obtained RBP4 level in plasma.
Verified Customer
Verified customer
Asked: 2019-08-11
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-08-11
Question
Q: Can RBP4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
C. Bhatt
Verified customer
Asked: 2019-05-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-26
Question
Q: if the enzyme conjugated RBP4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the RBP4 antigen?
Verified Customer
Verified customer
Asked: 2019-04-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-02-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-02-17
Question
Q: which procedure should I follow in order to thaw whole blood sample for RBP4 ELISA after freezing?
G. Adams
Verified customer
Asked: 2017-05-03
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test RBP4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-05-03
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-03-28
Question
Q: can I use citrate plasma as samples in Mouse RBP4 Picokine® ELISA Kit (Catalog # EK0832)?
Verified Customer
Verified customer
Asked: 2015-08-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of RBP4 causing disruption of its protein structure. RBP4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the RBP4 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-08-17
