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Product Info Summary
| SKU: | A03689-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-REN/Renin Antibody
SKU/Catalog Number
A03689-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-REN/Renin Antibody catalog # A03689-2. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-REN/Renin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03689-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human REN/Renin, which shares 95% and 95% amino acid (aa) sequence identity with mouse and rat REN/Renin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03689-2 is reactive to REN in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53-60 kDa
Calculated molecular weight
28461 MW
Background of Renin
This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03689-2 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
IHC: human renal cancer tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of REN/Renin using anti-REN/Renin antibody (A03689-2).
REN/Renin was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-REN/Renin Antibody (A03689-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For REN (Source: Uniprot.org, NCBI)
Gene Name
REN
Full Name
Renin
Weight
28461 MW
Superfamily
peptidase A1 family
Alternative Names
p-selectin glycoprotein ligand; Selplg; Psgl1 REN ADTKD4, HNFJ2, RTD renin renin|angiotensin-forming enzyme|angiotensinogenase|renin precursor, renal
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on REN, check out the REN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for REN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-REN/Renin Antibody (A03689-2)
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