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Product Info Summary
| SKU: | A04915 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-REC8 Antibody
SKU/Catalog Number
A04915
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-REC8 Antibody (Catalog # A04915). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-REC8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04915)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human REC8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04915 is reactive to REC8 in Human
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
62614 MW
Background of REC8
Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II By similarity.
Parisi S., Mol. Cell. Biol. 19:3515-3528(1999).
Ota T., Nat. Genet. 36:40-45(2004).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04915 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using REC8 antibody A04915.
Protein Target Info & Infographic
Gene/Protein Information For REC8 (Source: Uniprot.org, NCBI)
Gene Name
REC8
Full Name
Meiotic recombination protein REC8 homolog
Weight
62614 MW
Superfamily
rad21 family
Alternative Names
Meiotic recombination protein REC8 homolog;Cohesin Rec8p;REC8;REC8L1; REC8 HR21spBL1, Rec8p, REC8 REC8 meiotic recombination protein meiotic recombination protein REC8 homolog|REC8 homolog|REC8-like 1|cohesin Rec8p|cohesion rec8p|human homolog of rad21, S. pombe|kleisin-alpha|meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family|meiotic recombination protein REC8-like 1|recombination and sister chromatid cohesion protein homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on REC8, check out the REC8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for REC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-REC8 Antibody (A04915)
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No publications found for A04915
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-REC8 Antibody
Question
Does anti-REC8 antibody A04915 work on canine WB with lung?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Our lab technicians have not tested anti-REC8 antibody A04915 on canine. You can run a BLAST between canine and the immunogen sequence of anti-REC8 antibody A04915 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
My question regarding product A04915, anti-REC8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04915 anti-REC8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-11
Question
We are currently using anti-REC8 antibody A04915 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The anti-REC8 antibody (A04915) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-06
Question
Does anti-REC8 antibody A04915 work for WB with lung?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
According to the expression profile of lung, REC8 is highly expressed in lung. So, it is likely that anti-REC8 antibody A04915 will work for WB with lung.
Boster Scientific Support
Answered: 2020-01-02
Question
I was wanting to use to test anti-REC8 antibody A04915 on human lung for research purposes, then I may be interested in using anti-REC8 antibody A04915 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Zhang
Verified customer
Asked: 2019-10-03
Answer
The products we sell, including anti-REC8 antibody A04915, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-REC8 antibody A04915. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
Will A04915 anti-REC8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
You can see on the product datasheet, A04915 anti-REC8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-08
Question
Is this A04915 anti-REC8 antibody reactive to the isotypes of REC8?
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
The immunogen of A04915 anti-REC8 antibody is Synthesized peptide derived from internal of human REC8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-23
Question
I see that the anti-REC8 antibody A04915 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-09
Question
Is a blocking peptide available for product anti-REC8 antibody (A04915)?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
We do provide the blocking peptide for product anti-REC8 antibody (A04915). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-21
Question
I was wanting to use your anti-REC8 antibody for WB for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
J. Zhao
Verified customer
Asked: 2015-11-27
Answer
You can see on the product datasheet, A04915 anti-REC8 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-27
Question
See below the WB image, lot number and protocol we used for lung using anti-REC8 antibody A04915. Please let me know if you require anything else.
A. Zhao
Verified customer
Asked: 2015-10-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-16
Question
Is there a BSA free version of anti-REC8 antibody A04915 available?
S. Banerjee
Verified customer
Asked: 2013-02-28
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-REC8 antibody A04915 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-02-28
