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Product Info Summary
| SKU: | A06064-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RBP2 Antibody Picoband®
SKU/Catalog Number
A06064-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RBP2 Antibody Picoband® catalog # A06064-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RBP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06064-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RBP2 recombinant protein (Position: E10-K99).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06064-1 is reactive to RBP2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
80420 MW
Background of RBP2
RBP2(Retinol-binding protein 2) is a protein that in humans is encoded by the RBP2 gene. RBP2 is an abundant protein present in the small intestinal epithelium. It is though to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. RBP2 may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06064-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human SH-SY5Y whole cell, rat small intestines tissue, rat liver tissue, mouse small intestines tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RBP2 using anti-RBP2 antibody (A06064-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat small intestines tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse small intestines tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP2 antigen affinity purified polyclonal antibody (Catalog # A06064-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBP2 at approximately 16 kDa. The expected band size for RBP2 is at 16 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RBP2 (Source: Uniprot.org, NCBI)
Gene Name
RBP2
Full Name
Retinol-binding protein 2
Weight
80420 MW
Superfamily
calycin superfamily
Alternative Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1; ATP-dependent helicase 1; hHEL1; SMARCAD1; KIAA1122 RBP2 CRABP-II, CRBP2, CRBPII, RBPC2 retinol binding protein 2 retinol-binding protein 2|CRBP-II|cellular retinol-binding protein II|retinol binding protein 2, cellular
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBP2, check out the RBP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBP2 Antibody Picoband® (A06064-1)
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