Click image to see more details
Product Info Summary
| SKU: | RP1001 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IFN gamma Antibody Picoband®
SKU/Catalog Number
RP1001
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IFN gamma Antibody catalog # RP1001. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IFN gamma Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1001)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat IFN gamma recombinant protein (Position: 23-156).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1001 is reactive to Ifng in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
17918 MW
Background of IFN-gamma
Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1001 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure. Western blot analysis of IFN gamma using anti-IFN gamma antibody (RP1001).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane : Recombinant Rat IFN gamma Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN gamma antigen affinity purified polyclonal antibody (Catalog # RP1001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 22KD. The expected band size for IFN gamma is at 22KD.
Protein Target Info & Infographic
Gene/Protein Information For Ifng (Source: Uniprot.org, NCBI)
Gene Name
Ifng
Full Name
Interferon gamma
Weight
17918 MW
Superfamily
type II (or gamma) interferon family
Alternative Names
Interferon gamma;IFN-gamma;Ifng; IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ifng, check out the Ifng Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ifng: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IFN gamma Antibody Picoband® (RP1001)
Hello CJ!
RP1001 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Q,Sun J,Cao Y,Liu B,Li L,Mohammadtursun N,Zhang H,Dong J,Wu J.Bu-Shen-Fang-Chuan formula attenuates T-lymphocytes recruitment in the lung of rats with COPD through suppressing CXCL9/CXCL10/CXCL11-CXCR3 axis.Biomed Pharmacother.2020 Mar;123:109735.doi:1
Species: Rat
RP1001 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA
Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface
Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation
Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion
Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques
Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IFN gamma Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-IFN gamma Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-IFN gamma Antibody Picoband®
Question
Will anti-IFN gamma antibody RP1001 work on goat WB with blood?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Our lab technicians have not validated anti-IFN gamma antibody RP1001 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IFN gamma antibody RP1001 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
We are currently using anti-IFN gamma antibody RP1001 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
The anti-IFN gamma antibody (RP1001) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-31
Question
We ordered your anti-IFN gamma antibody for ELISA on leukocyte last year. I am using rat, and We are going to use the antibody for IHC next. I am looking for examining leukocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
I have checked the website and datasheets of our anti-IFN gamma antibody and it appears that RP1001 has been tested on rat in both ELISA and IHC. Thus RP1001 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-21
Question
Would anti-IFN gamma antibody RP1001 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
According to the expression profile of blood, IFNG is highly expressed in blood. So, it is likely that anti-IFN gamma antibody RP1001 will work for WB with blood.
Boster Scientific Support
Answered: 2019-10-01
Question
Would RP1001 anti-IFN gamma antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
It shows on the product datasheet, RP1001 anti-IFN gamma antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-19
Question
I see that the anti-IFN gamma antibody RP1001 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-25
Question
Is this RP1001 anti-IFN gamma antibody reactive to the isotypes of IFNG?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The immunogen of RP1001 anti-IFN gamma antibody is E. coli-derived rat IFN gamma recombinant protein(Position: 23-156). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-14
Question
We have observed staining in rat blood. What should we do? Is anti-IFN gamma antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
From what I have seen in literature blood does express IFNG. From what I have seen in Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-30
Question
See attached the WB image, lot number and protocol we used for blood using anti-IFN gamma antibody RP1001. Please let me know if you require anything else.
A. Anderson
Verified customer
Asked: 2018-12-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-03
Question
Is a blocking peptide available for product anti-IFN gamma antibody (RP1001)?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
We do provide the blocking peptide for product anti-IFN gamma antibody (RP1001). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-16
Question
I am looking for to test anti-IFN gamma antibody RP1001 on rat blood for research purposes, then I may be interested in using anti-IFN gamma antibody RP1001 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Jha
Verified customer
Asked: 2017-08-22
Answer
The products we sell, including anti-IFN gamma antibody RP1001, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-22
Question
Is there a BSA free version of anti-IFN gamma antibody RP1001 available?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IFN gamma antibody RP1001 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-20
Question
I was wanting to use your anti-IFN gamma antibody for WB for rat blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat blood identification?
M. Patel
Verified customer
Asked: 2017-02-28
Answer
As indicated on the product datasheet, RP1001 anti-IFN gamma antibody has been validated for ELISA, IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-28
Question
Our team were satisfied with the WB result of your anti-IFN gamma antibody. However we have observed positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
K. Miller
Verified customer
Asked: 2016-12-01
Answer
From what I have seen in literature, leukocyte does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-12-01
Question
My question regarding product RP1001, anti-IFN gamma antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Rodriguez
Verified customer
Asked: 2014-11-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1001 anti-IFN gamma antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-11-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IFN gamma antibody RP1001. Let me know if you need anything else.
A. Krishna
Verified customer
Asked: 2013-06-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-25
