Anti-RASSF7 Antibody Picoband™

Product Name

Anti-RASSF7 Antibody Picoband™

View all RASSF7 Antibodies

SKU/Catalog Number

A09402-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RASSF7 Antibody Picoband™ catalog # A09402-2. Tested in WB, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RASSF7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09402-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

E.coli-derived human RASSF7 recombinant protein (Position: R58-R342). Human RASSF7 shares 77.5% amino acid (aa) sequence identity with mouse RASSF7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09402-2 is reactive to RASSF7 in Human

Applications

A09402-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

52588 MW

Background of RASSF7

RASSF7 (Ras association domain-containing protein 7), also designated HRAS1-related cluster protein 1, is a 373 amino acid protein that localizes to the centrosome. RASSF7, RASSF8, RASSF9 and RASSF10 are four evolutionary conserved proteins that are collectively known as the N-terminal RASSF family. Expressed in skin, neural tube and eye, RASSF7 is believed to play an essential role in mitosis. Further supporting its mitotic role, knockdown of Xenopus RASSF7 has been shown to influence cell failure to form mitotic spindle and also cause nuclear breakdown and apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RASSF7 using anti-RASSF7 antibody (A09402-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASSF7 antigen affinity purified polyclonal antibody (Catalog # A09402-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASSF7 at approximately 37 kDa. The expected band size for RASSF7 is at 40 kDa.

Gene/Protein Information For RASSF7 (Source: Uniprot.org, NCBI)

Alternative Names

chromosome 11 open reading frame 13; HRAS1-related cluster protein 1; HRAS1-related cluster-1; HRC1C11orf13HRAS1; MGC126069; MGC126070; Ras association (RalGDS/AF-6) domain family (N-terminal) member 7; Ras association (RalGDS/AF-6) domain family 7; ras association domain-containing protein 7 RASSF7 C11orf13, CFAP88, FAP88, HRAS1, HRC1 Ras association domain family member 7 ras association domain-containing protein 7|HRAS1-related cluster protein 1|HRAS1-related cluster-1|Ras association (RalGDS/AF-6) domain family (N-terminal) member 7|Ras association (RalGDS/AF-6) domain family 7

For more info on RASSF7, check out the RASSF7 Infographic

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In this infographic, you will see the following information for RASSF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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