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Product Info Summary
| SKU: | A09402-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RASSF7 Antibody Picoband®
SKU/Catalog Number
A09402-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RASSF7 Antibody Picoband® catalog # A09402-2. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RASSF7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09402-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human RASSF7 recombinant protein (Position: R58-R342). Human RASSF7 shares 77.5% amino acid (aa) sequence identity with mouse RASSF7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09402-2 is reactive to RASSF7 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
37 kDa
Calculated molecular weight
52588 MW
Background of RASSF7
RASSF7 (Ras association domain-containing protein 7), also designated HRAS1-related cluster protein 1, is a 373 amino acid protein that localizes to the centrosome. RASSF7, RASSF8, RASSF9 and RASSF10 are four evolutionary conserved proteins that are collectively known as the N-terminal RASSF family. Expressed in skin, neural tube and eye, RASSF7 is believed to play an essential role in mitosis. Further supporting its mitotic role, knockdown of Xenopus RASSF7 has been shown to influence cell failure to form mitotic spindle and also cause nuclear breakdown and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09402-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HL-60 whole cell, human HepG2 whole cell, human Jurkat whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RASSF7 using anti-RASSF7 antibody (A09402-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASSF7 antigen affinity purified polyclonal antibody (Catalog # A09402-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASSF7 at approximately 37 kDa. The expected band size for RASSF7 is at 40 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RASSF7 (Source: Uniprot.org, NCBI)
Gene Name
RASSF7
Full Name
Ras association domain-containing protein 7
Weight
52588 MW
Alternative Names
70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody RASSF7 C11orf13, CFAP88, FAP88, HRAS1, HRC1 Ras association domain family member 7 ras association domain-containing protein 7|HRAS1-related cluster protein 1|HRAS1-related cluster-1|Ras association (RalGDS/AF-6) domain family (N-terminal) member 7|Ras association (RalGDS/AF-6) domain family 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RASSF7, check out the RASSF7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RASSF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RASSF7 Antibody Picoband® (A09402-2)
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