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Product Info Summary
| SKU: | A01463-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Raptor/RPTOR Antibody Picoband®
SKU/Catalog Number
A01463-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Raptor/RPTOR Antibody Picoband® catalog # A01463-3. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Raptor/RPTOR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01463-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Raptor/RPTOR recombinant protein (Position: R301-R358).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A01463-3 is reactive to RPTOR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
25722 MW
Background of RPTOR
Regulatory-associated protein of mTOR also known as raptor or KIAA1303 is an adapter protein that is encoded in humans by the RPTOR gene. This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01463-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human HepG2 whole cell, human Hela whole cell, human Mcf-7 whole cell, human Hek293 whole cell, human A549 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Raptor/RPTOR using anti-Raptor/RPTOR antibody (A01463-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Mcf-7 whole cell lysates,
Lane 4: human Hek293 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Raptor/RPTOR antigen affinity purified polyclonal antibody (Catalog # A01463-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Raptor/RPTOR at approximately 150KD. The expected band size for Raptor/RPTOR is at 150KD.
Protein Target Info & Infographic
Gene/Protein Information For RPTOR (Source: Uniprot.org, NCBI)
Gene Name
RPTOR
Full Name
Regulatory-associated protein of mTOR
Weight
25722 MW
Superfamily
WD repeat RAPTOR family
Alternative Names
Glutathione S-transferase A1; GST HA subunit 1; GST class-alpha member 1; GST-epsilon; GSTA1-1; GTH1; Glutathione S-transferase A1, N-terminally processed; GSTA1 RPTOR KOG1, Mip1 regulatory associated protein of MTOR complex 1 regulatory-associated protein of mTOR|p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|raptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPTOR, check out the RPTOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Raptor/RPTOR Antibody Picoband® (A01463-3)
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