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Product Info Summary
| SKU: | A01463 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
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Product info
Product Name
Anti-Raptor Rptor Antibody
SKU/Catalog Number
A01463
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Raptor Rptor Antibody (Catalog # A01463). Tested in ELISA, WB, ICC applications. This antibody reacts with Mouse.
Storage & Handling
Raptor antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Raptor Rptor Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01463)
Host
Rabbit
Contents
Raptor Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Raptor (CT) polyclonal antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of mouse Raptor. The immunogen is located within amino acids 1140 - 1190 of Raptor.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Raptor has multiple isoforms that may also be recognized by antibody.
Reactive Species
A01463 is reactive to RPTOR in Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
149471 MW
Background of RPTOR
The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation. These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels. Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif; mutation of this motif prevents the TOR-dependent phosphorylation of these proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01463 is guaranteed for ELISA, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Raptor in L1210 cell lysate with Raptor antibody at (A) 2 and (B) 4 μg/mL.
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Immunocytochemistry of Raptor in L1210 cells with Raptor antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RPTOR (Source: Uniprot.org, NCBI)
Gene Name
RPTOR
Full Name
Regulatory-associated protein of mTOR
Weight
149471 MW
Superfamily
WD repeat RAPTOR family
Alternative Names
Rap, Raptor, mKIAA1303, 4932417H02Rik, Regulatory-associated protein of mTOR RPTOR KOG1, Mip1 regulatory associated protein of MTOR complex 1 regulatory-associated protein of mTOR|p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|raptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPTOR, check out the RPTOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Raptor Rptor Antibody (A01463)
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