Product Info Summary
SKU: | M01463 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Raptor RPTOR Rabbit Monoclonal Antibody
SKU/Catalog Number
M01463
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Raptor RPTOR Rabbit Monoclonal Antibody catalog # M01463. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Raptor RPTOR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01463)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EGB-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Raptor
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01463 is reactive to RPTOR in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
149038 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01463 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:30
FC 1:30
Positive Control
WB: MCF-7 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Raptor expression in MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RPTOR (Source: Uniprot.org, NCBI)
Gene Name
RPTOR
Full Name
Regulatory-associated protein of mTOR
Weight
149038 MW
Superfamily
WD repeat RAPTOR family
Alternative Names
Regulatory-associated protein of mTOR;Raptor;p150 target of rapamycin (TOR)-scaffold protein;RPTOR;KIAA1303, RAPTOR; RPTOR KOG1, Mip1 regulatory associated protein of MTOR complex 1 regulatory-associated protein of mTOR|p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats|raptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPTOR, check out the RPTOR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Raptor RPTOR Rabbit Monoclonal Antibody (M01463)
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No publications found for M01463
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Customer Q&As
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5 Customer Q&As for Anti-Raptor RPTOR Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Raptor Rabbit Monoclonal antibody (M01463)?
Verified Customer
Verified customer
Asked: 2019-03-05
Answer
We do provide the blocking peptide for product anti-Raptor Rabbit Monoclonal antibody (M01463). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-03-05
Question
Does anti-Raptor Rabbit Monoclonal antibody M01463 work on horse IP with brain?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
Our lab technicians have not tested anti-Raptor Rabbit Monoclonal antibody M01463 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Raptor Rabbit Monoclonal antibody M01463 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Raptor Rabbit Monoclonal antibody M01463. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-24
Question
Would anti-Raptor Rabbit Monoclonal antibody M01463 work for IP with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
According to the expression profile of leukemic t-cell, RPTOR is highly expressed in leukemic t-cell. So, it is likely that anti-Raptor Rabbit Monoclonal antibody M01463 will work for IP with leukemic t-cell.
Boster Scientific Support
Answered: 2017-11-07
Question
We are currently using anti-Raptor Rabbit Monoclonal antibody M01463 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
K. Mangal
Verified customer
Asked: 2016-12-14
Answer
The anti-Raptor Rabbit Monoclonal antibody (M01463) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-14