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Product Info Summary
| SKU: | PB9413 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RANK/TNFRSF11A Antibody Picoband®
View all RANK/TNFRSF11A Antibodies
SKU/Catalog Number
PB9413
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RANK/TNFRSF11A Antibody Picoband® catalog # PB9413. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RANK/TNFRSF11A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9413)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse RANK recombinant protein (Position: C35-S214). Mouse RANK shares 95.6% amino acid (aa) sequence identity with rat RANK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9413 is reactive to Tnfrsf11a in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
66621 MW
Background of RANK/TNFRSF11A
Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is also expressed on dendritic cells and facilitates immune signaling.RANKL (Receptor Activator for Nuclear Factor κ B Ligand) is found on the surface of stromal cells, osteoblasts, and T cells. By analysis of somatic cell and radiation hybrid panels, Anderson et al. (1997) mapped the RANK gene to 18q22.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9413 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse , -
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: HEPA Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RANK using anti-RANK antibody (PB9413).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HEPA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANK antigen affinity purified polyclonal antibody (Catalog # PB9413) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANK at approximately 100 kDa. The expected band size for RANK is at 66 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf11a (Source: Uniprot.org, NCBI)
Gene Name
Tnfrsf11a
Full Name
Tumor necrosis factor receptor superfamily member 11A
Weight
66621 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 11A;Osteoclast differentiation factor receptor;ODFR;Receptor activator of NF-KB;CD265;Tnfrsf11a;Rank; TNFRSF11A CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TRANCER TNF receptor superfamily member 11a tumor necrosis factor receptor superfamily member 11A|Paget disease of bone 2|loss of heterozygosity, 18, chromosomal region 1|osteoclast differentiation factor receptor|receptor activator of NF-KB|receptor activator of nuclear factor-kappa B|tumor necrosis factor receptor superfamily, member 11a, NFKB activator
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnfrsf11a, check out the Tnfrsf11a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnfrsf11a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RANK/TNFRSF11A Antibody Picoband® (PB9413)
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No publications found for PB9413
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Customer Q&As
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1 Customer Q&As for Anti-RANK/TNFRSF11A Antibody Picoband®
Question
We are currently using anti-RANK/TNFRSF11A antibody PB9413 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on bovine tissues as well?
J. Krishna
Verified customer
Asked: 2016-02-16
Answer
The anti-RANK/TNFRSF11A antibody (PB9413) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-16
