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Product Info Summary
| SKU: | A09936 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Ran-binding protein 10 RANBP10 Antibody
SKU/Catalog Number
A09936
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ran-binding protein 10 RANBP10 Antibody (Catalog # A09936). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
RANBP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Ran-binding protein 10 RANBP10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09936)
Host
Rabbit
Contents
RANBP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RANBP10 antibody was raised against a 15 amino acid synthetic peptide near the center of human RANBP10. The immunogen is located within amino acids 380 - 430 of RANBP10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of RIM2 are known to exist. This antibody is predicted to have no cross-reactivity to other Rim proteins.
Reactive Species
A09936 is reactive to RANBP10 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
67257 MW
Background of RANBP10
The RAN binding protein 10 (RANBP10) is a cytoplasmic guanine nucleotide exchange factor that shares high sequence similarity to the MET-interacting protein RANBP9. RANBP10 is expressed in the cytoplasm of mature megakaryocytes (MKs) and platelets where it localizes to polymerized noncentrosomal microtubules. RANBP10 possesses guanine nucleotide exchange factor activity towards RAN, a GTPase involved in nucleocytoplasmic traffic, spindle formation at mitosis, and nuclear envelope assembly during telophase, suggesting that RANBP10 may also be involved in these activities. RANBP10 depletion in vitro caused the disturbance of polymerized filaments in MKs and platelets in RANBP10-null mice exhibited disorders in microtubule filament numbers and localization, indicating that RANBP10 may play a significant role in maintaining microtubule dynamics.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09936 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RANBP10 antibody can be used for detection of RANBP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RANBP10 in human skeletal muscle tissue lysate with RANBP10 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of RANBP10 in human skeletal muscle tissue with RANBP10 antibody at 5 μg/mL.
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Immunofluorescence of RANBP10 in mouse skeletal muscle tissue with RANBP10 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RANBP10 (Source: Uniprot.org, NCBI)
Gene Name
RANBP10
Full Name
Ran-binding protein 10
Weight
67257 MW
Superfamily
RANBP9/10 family
Alternative Names
KIAA1464, Ran-binding protein 10, RanBP10 RANBP10 RAN binding protein 10 ran-binding protein 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RANBP10, check out the RANBP10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RANBP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ran-binding protein 10 RANBP10 Antibody (A09936)
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