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Product Info Summary
| SKU: | M00204 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Ran Monoclonal Antibody
SKU/Catalog Number
M00204
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ran Monoclonal Antibody catalog # M00204. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ran Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00204)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACFI-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ran GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00204 is reactive to RAN in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
103 kDa
Calculated molecular weight
87710 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00204 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of Ran expression in (1)HeLa cell lysate; (2)RAW264.7 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For RAN (Source: Uniprot.org, NCBI)
Gene Name
RAN
Full Name
GTP-binding nuclear protein Ran
Weight
87710 MW
Superfamily
small GTPase superfamily
Alternative Names
Fibroblast growth factor receptor 3;FGFR-3;2.7.10.1;CD333;FGFR3;JTK4; RAN ARA24, Gsp1, TC4 RAN, member RAS oncogene family GTP-binding nuclear protein Ran|GTPase Ran|RanGTPase|androgen receptor-associated protein 24|guanosine triphosphatase Ran|member RAS oncogene family|ras-like protein TC4|ras-related nuclear protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAN, check out the RAN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ran Monoclonal Antibody (M00204)
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No publications found for M00204
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Customer Q&As
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4 Customer Q&As for Anti-Ran Monoclonal Antibody
Question
We are currently using anti-Ran Monoclonal antibody M00204 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
S. Jackson
Verified customer
Asked: 2020-04-30
Answer
The anti-Ran Monoclonal antibody (M00204) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
Question
We have been able to see staining in human erythroleukemia. What should we do? Is anti-Ran Monoclonal antibody supposed to stain erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
According to literature erythroleukemia does express RAN. According to Uniprot.org, RAN is expressed in testis, teratocarcinoma, brain, umbilical cord blood, hippocampus, lymph, ovary, skin uterus, platelet, brain cajal-retzius cell, colon adenocarcinoma, cervix carcinoma, lymphoblast, melanoma, erythroleukemia, liver, among other tissues. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2018-07-11
Question
We ordered your anti-Ran Monoclonal antibody for IF on brain cajal-retzius cell in the past. I am using rat, and We are going to use the antibody for WB next. My question regards examining brain cajal-retzius cell as well as skin uterus in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
I looked at the website and datasheets of our anti-Ran Monoclonal antibody and it appears that M00204 has been validated on rat in both IF and WB. Thus M00204 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-05
Question
My team were happy with the WB result of your anti-Ran Monoclonal antibody. However we have been able to see positive staining in hippocampus nucleus using this antibody. Is that expected? Could you tell me where is RAN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
According to literature, hippocampus does express RAN. Generally RAN expresses in nucleus. Regarding which tissues have RAN expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8421051, 10400640
Cervix carcinoma, Pubmed ID: 1961752
Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Lymph, Ovary, Skin, and Uterus, Pubmed ID: 15489334
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Teratocarcinoma, Pubmed ID: 2108320
Umbilical cord blood, Pubmed ID: 11042152
Boster Scientific Support
Answered: 2017-11-20
