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Product Info Summary
| SKU: | A06509-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-RAIDD CRADD Antibody
View all RAIDD/CRADD Antibodies
SKU/Catalog Number
A06509-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RAIDD CRADD Antibody (Catalog # A06509-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
RAIDD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RAIDD CRADD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06509-1)
Host
Rabbit
Contents
RAIDD Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RAIDD antibody was raised against a 19 amino acid peptide near the center of human RAIDD. The immunogen is located within amino acids 90 - 140 of RAIDD.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms.
Reactive Species
A06509-1 is reactive to CRADD in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
22745 MW
Background of RAIDD/CRADD
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1, 3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06509-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RAIDD antibody can be used for detection of RAIDD by Western blot at 0.5 - 1 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RAIDD in whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.
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Immunocytochemistry of RAIDD in HeLa cells with RAIDD antibody at 5 μg/mL.
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Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 μg/mL.
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Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CRADD (Source: Uniprot.org, NCBI)
Gene Name
CRADD
Full Name
Death domain-containing protein CRADD
Weight
22745 MW
Alternative Names
MRT34, RAIDD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain CRADD MRT34, RAIDD CASP2 and RIPK1 domain containing adaptor with death domain death domain-containing protein CRADD|CRADD/LYZ fusion|RIP-associated ICH1/CED3-homologous protein with death domain|caspase and RIP adaptor with death domain|death adaptor molecule RAIDD|death domain containing protein CRADD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRADD, check out the CRADD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRADD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAIDD CRADD Antibody (A06509-1)
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