Anti-RAD52 Antibody

Product Name

Anti-RAD52 Antibody

View all RAD52 Antibodies

SKU/Catalog Number

A01580

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-RAD52 Antibody (Catalog # A01580). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-RAD52 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01580)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 350-375 of the Human Rad 52 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01580 is reactive to RAD52 in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

46169 MW

Background of RAD52

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01580 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:20,000
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

Gene/Protein Information For RAD52 (Source: Uniprot.org, NCBI)

Alternative Names

RAD52, RAD-52, RAD 52, DNA repair protein RAD52 homolog, RAD 52, RAD52 homolog, recombination protein RAD52 RAD52 RAD52 homolog, DNA repair protein DNA repair protein RAD52 homolog|recombination protein RAD52|rhabdomyosarcoma MU-RMS-40.23

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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