Anti-RAD21 Antibody

Product Name

Anti-RAD21 Antibody

View all RAD21 Antibodies

SKU/Catalog Number

A01864

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RAD21 Antibody (Catalog # A01864). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RAD21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01864)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RAD21.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01864 is reactive to RAD21 in Human, Mouse

Reconstitution

Calculated molecular weight

71690 MW

Background of RAD21

Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.

McKay M.J., Genomics 36:305-315(1996).
Sadano H., Biochem. Biophys. Res. Commun. 267:418-422(2000).
Nomura N., DNA Res. 1:223-229(1994).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01864 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For RAD21 (Source: Uniprot.org, NCBI)

Alternative Names

Double-strand-break repair protein rad21 homolog;hHR21;Nuclear matrix protein 1;NXP-1;SCC1 homolog;RAD21;HR21, KIAA0078, NXP1; RAD21 CDLS4, HR21, HRAD21, MCD1, MGS, NXP1, SCC1, hHR21 RAD21 cohesin complex component double-strand-break repair protein rad21 homolog|NXP-1|RAD21 homolog|SCC1 homolog|kleisin|nuclear matrix protein 1|protein involved in DNA double-strand break repair|sister chromatid cohesion 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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