Product Info Summary
SKU: | M05768 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Rab9 RAB9A Rabbit Monoclonal Antibody
SKU/Catalog Number
M05768
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rab9 RAB9A Rabbit Monoclonal Antibody catalog # M05768. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab9 RAB9A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05768)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DIA-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rab9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05768 is reactive to RAB9A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
22838 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05768 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Rab9 expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RAB9A (Source: Uniprot.org, NCBI)
Gene Name
RAB9A
Full Name
Ras-related protein Rab-9A
Weight
22838 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-9A;RAB9A;RAB9; RAB9A RAB9 RAB9A, member RAS oncogene family ras-related protein Rab-9A|RAB9, member RAS oncogene family
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB9A, check out the RAB9A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB9A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab9 RAB9A Rabbit Monoclonal Antibody (M05768)
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Customer Q&As
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4 Customer Q&As for Anti-Rab9 RAB9A Rabbit Monoclonal Antibody
Question
Would anti-Rab9 Rabbit Monoclonal antibody M05768 work on monkey WB with amniotic fluid?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Our lab technicians have not tested anti-Rab9 Rabbit Monoclonal antibody M05768 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Rab9 Rabbit Monoclonal antibody M05768 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey amniotic fluid in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-03
Question
Is a blocking peptide available for product anti-Rab9 Rabbit Monoclonal antibody (M05768)?
D. Johnson
Verified customer
Asked: 2018-09-05
Answer
We do provide the blocking peptide for product anti-Rab9 Rabbit Monoclonal antibody (M05768). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-05
Question
My question regarding product M05768, anti-Rab9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M05768 anti-Rab9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amniotic fluid using anti-Rab9 Rabbit Monoclonal antibody M05768. Let me know if you need anything else.
C. Parker
Verified customer
Asked: 2015-05-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-22