Product Info Summary
SKU: | PA1436 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Annexin VI/ANXA6 Antibody
View all Annexin A6 Antibodies
SKU/Catalog Number
PA1436
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin VI/ANXA6 Antibody catalog # PA1436. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin VI/ANXA6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1436)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VI, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1436 is reactive to ANXA6 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
75873 MW
Background of Annexin A6
Annexin A6 (ANXA6) is a member of a family of proteins that bind membrane or cytoskeleton in a Ca (2+)-dependent manner. These proteins are characterized by homologous amino acid sequences that are present in multiple copies in each protein. ANXA6 gene is assigned to 5q32-q34 by use of a cDNA clone to probe genomic DNA from rodent-human somatic cell hybrids and for in situ hybridization.The ANX6 gene is approximately 60 kb long and contains 26 exons. The genomic sequence at the 3-prime end does not contain a canonical polyadenylylation signal.Ca (2+)-dependent binding between CRHSP28 and ANXA6 is required for acinar cell membrane trafficking events and digestive enzyme secretion
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1436 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Ovary Tissue, Rat Liver Tissue, Rat Intestinum Tenue Tissue, Rat Brain Tissue, A549 Cell, JURKAT Cell, RAJI Cell, CEM Cell, SMMC Cell
IHC: Rat Spleen Tissue
IHC-F: Rat Spleen Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Annexin VI antibody, PA1436, Western blotting
Lane 1: Rat Ovary Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Intestinum Tenue Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: A549 Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: RAJI Cell Lysate
Lane 8: CEM Cell Lysate
Lane 9: SMMC Cell Lysate
Click image to see more details
Anti-Annexin VI antibody, PA1436, IHC(P)
IHC(P): Rat Spleen Tissue
Click image to see more details
Anti-Annexin VI antibody, PA1436, IHC(F)
IHC(F): Rat Spleen Tissue
Protein Target Info & Infographic
Gene/Protein Information For ANXA6 (Source: Uniprot.org, NCBI)
Gene Name
ANXA6
Full Name
Annexin A6
Weight
75873 MW
Superfamily
annexin family
Alternative Names
67 kDa calelectrin; Annexin A6; annexin VI (p68); Annexin VI; Annexin-6; ANX6; ANXA6; calcium-binding protein p68; Calelectrin; calphobindin II; calphobindin-II; CBP68; chromobindin-20; CPB-II ; CPB-II; Lipocortin VI; p68; p70; Protein III ANXA6 ANX6, CBP68, CPB-II, p68, p70 annexin A6 annexin A6|67 kDa calelectrin|annexin VI (p68)|annexin-6|calcium-binding protein p68|calelectrin|calphobindin II|chromobindin-20|lipocortin VI|testis secretory sperm-binding protein Li 198a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANXA6, check out the ANXA6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANXA6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Annexin VI/ANXA6 Antibody (PA1436)
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Customer Q&As
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3 Customer Q&As for Anti-Annexin VI/ANXA6 Antibody
Question
Our lab want to know about to test anti-Annexin VI/ANXA6 antibody PA1436 on rat liver for research purposes, then I may be interested in using anti-Annexin VI/ANXA6 antibody PA1436 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Roberts
Verified customer
Asked: 2020-03-05
Answer
The products we sell, including anti-Annexin VI/ANXA6 antibody PA1436, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-05
Question
Will PA1436 anti-Annexin VI/ANXA6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
It shows on the product datasheet, PA1436 anti-Annexin VI/ANXA6 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-21
Question
We are currently using anti-Annexin VI/ANXA6 antibody PA1436 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
D. Gonzalez
Verified customer
Asked: 2013-05-02
Answer
The anti-Annexin VI/ANXA6 antibody (PA1436) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-02