Click image to see more details
Product Info Summary
| SKU: | PB9884 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Rab9/RAB9A Antibody Picoband®
SKU/Catalog Number
PB9884
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rab9/RAB9A Antibody Picoband® catalog # PB9884. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab9/RAB9A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9884)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab9, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9884 is reactive to RAB9A in Human, Monkey, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
22838 MW
Background of RAB9A
Ras-related protein Rab-9A, also called RAB9, is a protein that in humans is encoded by the RAB9A gene. This gene is mapped to Xp22.2. RAB9 has been localized to components of the endocytic/exocytic pathway and has been implicated in recycling of membrane receptors. It has been found that downregulation of RAB9A gene expression in HeLa cells induced severe cell vacuolation. RAB9A GTPase is directly bound by TIP47 in its active, GTP-bound conformation. Moreover, RAB9A increases the affinity of TIP47 for its cargo. What’s more, this gene may involved in the transport of proteins between the endosomes and the trans Golgi network. RAB9A has been shown to interact with RABEPK and TIP47.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9884 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human K562 whole cell, human HepG2 whole cell, human 293T whole cell, monkey COS-7 whole cell, mouse NIH/3T3 whole cell
ICC/IF: Hela cell
FCM: U20S cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (PB9884).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab9/RAB9A antigen affinity purified polyclonal antibody (Catalog # PB9884) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab9/RAB9A at approximately 23 kDa. The expected band size for Rab9/RAB9A is at 23 kDa.
Click image to see more details
Figure 2. IF analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (PB9884).
Rab9/RAB9A was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Rab9/RAB9A Antibody (PB9884) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of U20S cells using anti-Rab9/RAB9A antibody (PB9884).
Overlay histogram showing U20S cells stained with PB9884 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rab9/RAB9A Antibody (PB9884, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RAB9A (Source: Uniprot.org, NCBI)
Gene Name
RAB9A
Full Name
Ras-related protein Rab-9A
Weight
22838 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-9A;RAB9A;RAB9; RAB9A RAB9 RAB9A, member RAS oncogene family ras-related protein Rab-9A|RAB9, member RAS oncogene family
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB9A, check out the RAB9A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB9A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab9/RAB9A Antibody Picoband® (PB9884)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Rab9/RAB9A Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Rab9/RAB9A Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Rab9/RAB9A Antibody Picoband®
Question
We are currently using anti-Rab9/RAB9A antibody PB9884 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-Rab9/RAB9A antibody (PB9884) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
