Click image to see more details
Product Info Summary
| SKU: | A14616-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RAB36 Antibody Picoband®
SKU/Catalog Number
A14616-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAB36 Antibody Picoband® catalog # A14616-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RAB36 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14616-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RAB36 recombinant protein (Position: Q56-E323).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A14616-1 is reactive to RAB36 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
64099 MW
Background of RAB36
Ras-related protein Rab-36 is a protein that in humans is encoded by the RAB36 gene. The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies. Increasing data suggests an important role for Rab proteins in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. Rab 36 is a ubiquitously expressed member of the Rab family of proteins and localizes to the Golgi membrane where it is believed to participate in protein transport. The gene encoding Rab 36 localizes to a critical region of chromosome 22 that is often deleted or involved in translocations in malignant rhabdoid tumors (MRTs). This suggests that Rab 36 may play a role in the development of MRTs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14616-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, rat testis tissue, rat PC-12 whole cell, mouse ovary tissue, mouse testis tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RAB36 using anti-RAB36 antibody (A14616-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mouse ovary tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB36 antigen affinity purified polyclonal antibody (Catalog # A14616-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB36 at approximately 36 kDa. The expected band size for RAB36 is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RAB36 (Source: Uniprot.org, NCBI)
Gene Name
RAB36
Full Name
Ras-related protein Rab-36
Weight
64099 MW
Superfamily
small GTPase superfamily
Alternative Names
DAP3-binding cell death enhancer 1; Death ligand signal enhancer; DELE1; DELE; KIAA0141 RAB36 RAB36, member RAS oncogene family ras-related protein Rab-36|small GTP-binding protein Rab36
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB36, check out the RAB36 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB36: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB36 Antibody Picoband® (A14616-1)
Hello CJ!
No publications found for A14616-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RAB36 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-RAB36 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
