Anti-R-spondin-1 RSPO1 Antibody

Product Name

Anti-R-spondin-1 RSPO1 Antibody

View all R-Spondin 1 Antibodies

SKU/Catalog Number

A02857-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-R-spondin-1 RSPO1 Antibody (Catalog # A02857-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

RSPO1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-R-spondin-1 RSPO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02857-1)

Host

Rabbit

Contents

RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-RSPO1 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human RSPO1. The immunogen is located within the first 50 amino acids of RSPO1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.

Reactive Species

A02857-1 is reactive to RSPO1 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

28959 MW

Background of R-Spondin 1

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02857-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.5-2 μg/mL; IHC: 2.5-5 μg/mL; IF: 5 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For RSPO1 (Source: Uniprot.org, NCBI)

Alternative Names

RSPO, CRISTIN3, R-spondin-1, Roof plate-specific spondin-1, hRspo1 Rspo1|R, R-, R-spondin, Rspondin|R-spondin 1|R-spondin-1|R-spondin homolog|cristin-3|cysteine-rich and single thrombospondin domain-containing protein 3|mCristin-3|roof plate-specific spondin-1|thrombospondin type 1 domain containing gene Rspondin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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