Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody

Product Name

Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody

View all Pyruvate Dehydrogenase E1 beta subunit Antibodies

SKU/Catalog Number

M03998

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody catalog # M03998. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03998)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEAD-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03998 is reactive to PDHB in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

85 kDa

Calculated molecular weight

50112 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M03998 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Positive Control

WB: HeLa cell

Validation Images & Assay Conditions

Gene/Protein Information For PDHB (Source: Uniprot.org, NCBI)

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 5;MAP kinase kinase 5;MAPKK 5;2.7.12.2;MAPK/ERK kinase 5;MEK 5;MAP2K5;MEK5, MKK5, PRKMK5; PDHB PDHBD, PDHE1-B, PDHE1B, PHE1B pyruvate dehydrogenase E1 subunit beta pyruvate dehydrogenase E1 component subunit beta, mitochondrial|epididymis secretory sperm binding protein|pyruvate dehydrogenase (lipoamide) beta|pyruvate dehydrogenase E1 beta subunit|pyruvate dehydrogenase, E1 beta polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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