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Product Info Summary
| SKU: | A07555-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PXK Antibody Picoband®
SKU/Catalog Number
A07555-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PXK Antibody Picoband® catalog # A07555-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PXK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07555-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PXK recombinant protein (Position: E5-K576).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07555-1 is reactive to PXK in Human, Monkey
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
157578 MW
Background of PXK
PX domain containing serine/threonine kinase also known as PXK is a protein which in humans is encoded by the PXK gene. This gene encodes a phox (PX) domain-containing protein which may be involved in synaptic transmission and the ligand-induced internalization and degradation of epidermal growth factors. Variations in this gene may be associated with susceptibility to systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07555-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, monkey COS-7 whole cell
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PXK using anti-PXK antibody (A07555-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXK antigen affinity purified polyclonal antibody (Catalog # A07555-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PXK at approximately 65 kDa. The expected band size for PXK is at 65 kDa.
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Figure 2. IF analysis of PXK using anti-PXK antibody (A07555-1).
PXK was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PXK Antibody (A07555-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PXK (Source: Uniprot.org, NCBI)
Gene Name
PXK
Full Name
PX domain-containing protein kinase-like protein
Weight
157578 MW
Superfamily
protein kinase superfamily
Alternative Names
C-C motif chemokine 16; Chemokine CC-4; HCC-4; Chemokine LEC; IL-10-inducible chemokine; LCC-1; Liver-expressed chemokine; Lymphocyte and monocyte chemoattractant; LMC; Monotactin-1; MTN-1; NCC-4; Small-inducible cytokine A16; CCL16; ILINCK; NCC4; SCYA16 PXK MONAKA, SLOB PX domain containing serine/threonine kinase like PX domain-containing protein kinase-like protein|PX ser/thr kinase v2|PX serine/threonine kinase|modulator of Na,K-ATPase long form
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PXK, check out the PXK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PXK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PXK Antibody Picoband® (A07555-1)
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