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Product Info Summary
| SKU: | PA1313 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PUMA/BBC3 Antibody Picoband®
SKU/Catalog Number
PA1313
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PUMA/BBC3 Antibody catalog # PA1313. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PUMA/BBC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1313)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1313 is reactive to BBC3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
20532 MW
Background of PUMA
The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1313 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: HELA Whole Cell, Rat Kidney Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-PUMA antibody, PA1313, Western blotting
All lanes: Anti PUMA(PA1313) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 21KD
Observed bind size: 21KD
Protein Target Info & Infographic
Gene/Protein Information For BBC3 (Source: Uniprot.org, NCBI)
Gene Name
BBC3
Full Name
Bcl-2-binding component 3, isoforms 3/4
Weight
20532 MW
Alternative Names
Bcl-2-binding component 3;JFY-1;p53 up-regulated modulator of apoptosis;BBC3;PUMA; BBC3 JFY-1, JFY1, PUMA BCL2 binding component 3 bcl-2-binding component 3|p53 up-regulated modulator of apoptosis|p53-upregulated modulator of apoptosis
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BBC3, check out the BBC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BBC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PUMA/BBC3 Antibody Picoband® (PA1313)
Hello CJ!
PA1313 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nerve growth factor reduces apoptotic cell death in rat facial motor neurons after facial nerve injury
Zhao Y, Wu S, Wu J, Jia P, Gao S, Yan X, Wang Y. Cancer Biol Ther. 2011 Jan 1;11(1):95-107. Doi: 10.4161/Cbt.11.1.13960. Introduction Of Hypoxia-Targeting P53 Fusion Protein For The Selective Therapy Of Non-Small Cell Lung Cancer.
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-PUMA/BBC3 Antibody Picoband®
Question
We are currently using anti-PUMA/BBC3 antibody PA1313 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The anti-PUMA/BBC3 antibody (PA1313) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
Is this PA1313 anti-PUMA/BBC3 antibody reactive to the isotypes of BBC3?
P. Krishna
Verified customer
Asked: 2015-02-16
Answer
The immunogen of PA1313 anti-PUMA/BBC3 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA(145-159aa ADDLNAQYERRRQEE), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-16
Question
Does PA1313 anti-PUMA/BBC3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Anderson
Verified customer
Asked: 2014-02-03
Answer
You can see on the product datasheet, PA1313 anti-PUMA/BBC3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-03
