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Product Info Summary
| SKU: | A10343-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-PTH2R Antibody Picoband®
SKU/Catalog Number
A10343-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTH2R Antibody Picoband® catalog # A10343-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PTH2R Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10343-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PTH2R recombinant protein (Position: A22-E511).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10343-1 is reactive to PTH2R in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
41409 MW
Background of PTH2R
Parathyroid hormone 2 receptor is a protein that in humans is encoded by the PTH2R gene. The protein encoded by this gene is a member of the G-protein coupled receptor 2 family. This protein is a receptor for parathyroid hormone (PTH). This receptor is more selective in ligand recognition and has a more specific tissue distribution compared to parathyroid hormone receptor 1 (PTHR1). It is activated only by PTH and not by parathyroid hormone-like hormone (PTHLH) and is particularly abundant in brain and pancreas. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10343-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HEL whole cell, human SH-SY5Y whole cell
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTH2R using anti-PTH2R antibody (A10343-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTH2R antigen affinity purified polyclonal antibody (Catalog # A10343-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTH2R at approximately 75 kDa. The expected band size for PTH2R is at 62 kDa.
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Figure 2. IF analysis of PTH2R using anti-PTH2R antibody (A10343-1).
PTH2R was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTH2R Antibody (A10343-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PTH2R (Source: Uniprot.org, NCBI)
Gene Name
PTH2R
Full Name
Parathyroid hormone 2 receptor
Weight
41409 MW
Superfamily
G-protein coupled receptor 2 family
Alternative Names
Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA PTH2R PTHR2 parathyroid hormone 2 receptor parathyroid hormone 2 receptor|PTH2 receptor|parathyroid hormone receptor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTH2R, check out the PTH2R Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTH2R: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTH2R Antibody Picoband® (A10343-1)
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