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Product Info Summary
| SKU: | A03891-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PTF1A Antibody Picoband®
SKU/Catalog Number
A03891-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTF1A Antibody Picoband® catalog # A03891-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTF1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03891-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PTF1A recombinant protein (Position: M1-Q292).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03891-2 is reactive to PTF1A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
35.185kDa
Background of Ptf1a
Pancreas transcription factor 1 subunit alpha is a protein that in humans is encoded by the PTF1A gene. It is mapped to 10p12.2. This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03891-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat pancreas tissue, mouse pancreas tissue, human PANC-1 whole cell, human PANC-1 whole cell, human U2OS whole cell, mouse SP2-0 whole cell
ICC/IF: A431 cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTF1A using anti-PTF1A antibody (A03891-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: mouse pancreas tissue lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human U2OS whole cell lysates,
Lane 6: mouse SP2-0 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTF1A antigen affinity purified polyclonal antibody (Catalog # A03891-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTF1A at approximately 48KD. The expected band size for PTF1A is at 35KD.
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Figure 2. IF analysis of PTF1A using anti-PTF1A antibody (A03891-2).
PTF1A was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PTF1A Antibody (A03891-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A431 cells using anti-PTF1A antibody (A03891-2).
Overlay histogram showing A431 cells stained with A03891-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTF1A Antibody (A03891-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PTF1A (Source: Uniprot.org, NCBI)
Gene Name
PTF1A
Full Name
Pancreas transcription factor 1 subunit alpha
Weight
35.185kDa
Alternative Names
Pancreas transcription factor 1 subunit alpha; Class A basic helix-loop-helix protein 29; bHLHa29; Pancreas-specific transcription factor 1a; bHLH transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; PTF1-p48; PTF1A; BHLHA29; PTF1P48 Ptf1a|PTF1-p, PTF1-p48, PTF1p48, bHLHa, bHLHa29|pancreas specific transcription factor, 1a|pancreas transcription factor 1 subunit alpha|bHLH transcription factor p48|p48 DNA-binding subunit of transcription factor PTF1|pancreas transcription factor1 p48 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTF1A, check out the PTF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTF1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTF1A Antibody Picoband® (A03891-2)
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